Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF

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Recombinant Mouse CLEC4B2 Fc Chimera (Catalog # 10087-DC) binds fluorescein-conjugated S. aureus bioparticles. The ED50 for this effect is 0.25-1.25 μg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834. The ED50 for this effect is 0.25-1.25 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CLEC4B2/mDCAR1 protein
MDPMouse IgG2A
(Glu98-Lys330)
IEGR Mouse CLEC4B2
(Gln42-Leu208)
Accession # Q67DU8
N-terminusC-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-59 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF

  • CLEC4B2
  • mDCAR1

Background

CLEC4B2 (C-type Lectin domain family 4, member b2), also called dendritic cell immunoactivating receptor 1 (DCAR1) and antigen presenting cell lectin-like receptor A1 (APLRA1), is a type II membrane protein belonging to the cell surface C-type lectin domain family (1, 2). Mouse CLEC4B2 is synthesized as a 208 amino acid (aa) precursor that contains a 22 aa transmembrane segment and a 167 aa extracellular domain (ECD) containing a C-type lectin domain. The ECD of mouse CLEC4B2 shares 71% and 50% aa identity with rat CLEC4B2 ECD and human CLEC4B2 ECD, respectively. Activation of CLEC4B2 in the epidermis regulates immune defenses. CLEC4B2 potentially has a second role in host defense. CLEC4B2 is specifically required for the innate immune response to fungal infection and wounding and place this GPCR further upstream than any other known component of innate immune recognition and signaling in C. elegans epidermis.
  1. Flornes, L. M. et al. (2004) Immunogenetics 56:506.
  2. Kaden, S. A. et al. (2009) J. Immunol. 183:5069.
  3. Zugasti, O. et al. (2014) Nat. Immunol. 15:833.

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