Recombinant Human Wnt/RSPO2 Agonist Protein, CF

Recombinant Human Wnt/RSPO2 Agonist Protein (Catalog # BT-WRSP2) activates TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 6.00‑72.0 ng/mL.

2 μg/lane of Recombinant Human Wnt/RSPO2 Agonist Protein (Catalog # BT-WRSP2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-97 & >190 kDa under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Wnt/RSPO2 Agonist Protein, CF Summary

• Details of Functionality: Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 6.00‑72.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Wnt/RSPO2 protein
  Proprietary
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• SDS-PAGE: 83-97 kDa & >190 kDa under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt/RSPO2 Agonist Protein, CF

• CRISTIN2
• HHRRD
• roof plate-specific spondin-2
• R-spondin 2 homolog
• R-spondin-2
• TETAMS2
• Wnt
• Wnt/RSPO2

Background

Wnt signaling is known to promote self-renewal of adult stem cells. R-Spondins amplify Wnt signaling in adult stem cells by binding to LGR5 receptors resulting in increased levels of Wnt receptor localization at the membrane surface. Many current organoid protocols include addition of Wnt-3a and R-Spondin proteins to the media for optimal adult stem cell maintenance and health of organoids. Fusion proteins known known as Wnt Surrogates have been described that have enhanced solubility when compared to the widely used Wnt-3a protein and mirror the activities of Wnt signaling.¹ R&D Systems has constructed recombinant fusion proteins designed to bind elements of the Wnt receptor system including LGR-5, Frizzled, and LRP-5/6. These can initiate canonical Wnt signaling and are designed to simplify cell culture protocols by substituting for conditioned media or the addition of recombinant Wnt and R-Spondin.

1. Janda et al. (2017) Nature 545:234.