Recombinant Human LRRC52 Fc Chimera Protein, CF

2 μg/lane of Recombinant Human LRRC52 Fc Chimera (Catalog # 10260-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 120 -140 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LRRC52 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived human LRRC52 protein
  

  Human LRRC-52 (Ser24-Asp244) Accession # Q8N7C0	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q8N7C0
• N-terminal Sequence: Ser24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-80 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRRC52 Fc Chimera Protein, CF

• BK Channel Auxiliary Gamma Subunit LRRC52
• FLJ25811
• leucine rich repeat containing 52
• leucine-rich repeat-containing protein 52
• LRRC52

Background

LRRC52 (Leucine-rich repeat-containing protein 52) belongs to an extracellular leucine-rich-repeat–only (Elron) cluster, which includes five other LRRC proteins: LRRC26, LRRC38, LRRC55, LRTM1 and LRTM2 (1). Elron cluster members are type I transmembrane proteins with an extracellular domain (ECD) containing several LRR motifs and a cytoplasmic C-terminal tail with a short stretch of acidic residues (1). The mature ECD of LRRC52 is 221 amino acids (aa), containing 5 LRR motifs, and shares 76% and 78% identity with mouse and rat LRRC52, respectively. LRRC-52 is dominantly expressed in testis and skeletal muscle and is also detected in several other tissues including placenta, kidney, lung, and some glands (2, 3). LRRC52 has been identified as a testes specific SLO3 K⁺ channel accessory protein in mouse (2). LRRC52 is proposed to be essential for alkalization dependent activation of the KSper current at physiological membrane potentials and pH (2). Activation of the SLO3 channel is critical in the fertilization process and loss of function results in infertility (2, 4, 5). A second Elron cluster protein, LRRC26, was recently identified as a regulatory subunit of SLO1 BK-type K⁺ channels, suggesting a general function for the entire family (6). Based on function, LRRC52 might be a therapeutic target for male contraception (7).

1. Dolan, J. et al. (2007) BMC Genomics 8:320.
2. Yang, C. et al. (2011) PNAS 108:19419.
3. Leonetti, M. et al. (2012) PNAS. 109:19274.
4. Navarro, B. et al. (2007) PNAS 104:7688.
5. Sanit, C. et al. (2010) FEBS Lett. 584:1041.
6. Yan, J. and Aldrich, RW. (2010) Nature 466:513.
7. Zeng, XH. et al. (2015) PNAS 112:2599.

Bioinformatics

• Uniprot:
  • Q8N7C0()