Recombinant Human LRRC52 Fc Chimera Protein, CF

Images

 
2 μg/lane of Recombinant Human LRRC52 Fc Chimera (Catalog # 10260-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human LRRC52 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human LRRC52 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human LRRC52 protein
Human LRRC-52
(Ser24-Asp244)
Accession # Q8N7C0
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Ser24
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-80 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRRC52 Fc Chimera Protein, CF

  • BK Channel Auxiliary Gamma Subunit LRRC52
  • FLJ25811
  • leucine rich repeat containing 52
  • leucine-rich repeat-containing protein 52
  • LRRC52

Background

LRRC52 (Leucine-rich repeat-containing protein 52) belongs to an extracellular leucine-rich-repeat–only (Elron) cluster, which includes five other LRRC proteins: LRRC26, LRRC38, LRRC55, LRTM1 and LRTM2 (1). Elron cluster members are type I transmembrane proteins with an extracellular domain (ECD) containing several LRR motifs and a cytoplasmic C-terminal tail with a short stretch of acidic residues (1). The mature ECD of LRRC52 is 221 amino acids (aa), containing 5 LRR motifs, and shares 76% and 78% identity with mouse and rat LRRC52, respectively. LRRC-52 is dominantly expressed in testis and skeletal muscle and is also detected in several other tissues including placenta, kidney, lung, and some glands (2, 3). LRRC52 has been identified as a testes specific SLO3 K+ channel accessory protein in mouse (2). LRRC52 is proposed to be essential for alkalization dependent activation of the KSper current at physiological membrane potentials and pH (2). Activation of the SLO3 channel is critical in the fertilization process and loss of function results in infertility (2, 4, 5). A second Elron cluster protein, LRRC26, was recently identified as a regulatory subunit of SLO1 BK-type K+ channels, suggesting a general function for the entire family (6). Based on function, LRRC52 might be a therapeutic target for male contraception (7).
  1. Dolan, J. et al. (2007) BMC Genomics 8:320.
  2. Yang, C. et al. (2011) PNAS 108:19419.
  3. Leonetti, M. et al. (2012) PNAS. 109:19274.
  4. Navarro, B. et al. (2007) PNAS 104:7688.
  5. Sanit, C. et al. (2010) FEBS Lett. 584:1041.
  6. Yan, J. and Aldrich, RW. (2010) Nature 466:513.
  7. Zeng, XH. et al. (2015) PNAS 112:2599.

Customers Who Viewed This Item Also Viewed...

NBP2-33726
Species: Hu
Applications: IHC, IHC-P
NBP2-81900
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP3-05611
Species: Rt
Applications: WB
NBP3-05611
Species: Rt
Applications: WB
NBP2-76945
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-90986
Species: Hu
Applications: IHC, IHC-P
10260-LR
Species: Hu
Applications: Bioactivity

Publications for LRRC52 (10260-LR) (0)

There are no publications for LRRC52 (10260-LR).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LRRC52 (10260-LR) (0)

There are no reviews for LRRC52 (10260-LR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for LRRC52 (10260-LR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional LRRC52 Products

Blogs on LRRC52

There are no specific blogs for LRRC52, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human LRRC52 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot