Recombinant Human IgE Fc His-tag Protein, CF

Measured by its binding ability in a functional ELISA. Recombinant Human IgE Fc His-tag Protein (Catalog # 11647-IE) binds to Recombinant Human Fc epsilon RI alpha Fc Chimera Protein ( 11561-FC) with an ED50 of 0.100-1.00 ng/mL.

2 μg/lane of Recombinant Human IgE Fc His-tag Protein (Catalog # 11647-IE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40‑56 kDa, under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IgE Fc His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human IgE Fc His-tag binds to Recombinant Human Fc epsilon RI alpha Fc Chimera Protein (Catalog # 11561-FC) with an ED₅₀ of 0.100-1.00 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IgE Fc protein
  Ser106-Lys428, with an N-terminal 6-His tag
• Accession #: P01854-1
• N-terminal Sequence: His
• Structure / Form: Disulfide linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-56 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IgE Fc His-tag Protein, CF

• constant region of heavy chain of IgE
• IgE Fc
• immunoglobulin epsilon
• immunoglobulin heavy constant epsilon

Background

IgE is an immunoglobulin that plays a critical role in the immune response to parasitic infections and in the development of allergic reactions. Like other antibody classes, IgE has the same molecular architecture that includes two heavy chains and two light chains, but its heavy chain contains an additional domain compared to IgG heavy chain. IgE binds to its receptor, Fc epsilon RI, on the surface of mast cells and basophils, triggering the release of histamine and other mediators that cause the symptoms of allergic reactions (1, 2). The recombinant human IgE-Fc protein is a valuable tool for researchers studying the mechanisms of allergic reactions and the development of new therapies for allergic diseases. It can be used in a wide range of applications, including ELISA assays, Western blotting, functional assays to study the binding of IgE to its receptor, Fc epsilon RI, and the downstream signaling pathways that are activated upon binding (1, 2, 3). In addition to its research applications, the recombinant human IgE-Fc protein has potential therapeutic applications in the treatment of allergic diseases. Therapies that target IgE have been shown to be effective in the treatment of allergic diseases such as asthma and atopic dermatitis (4).

1. Gould, H. J. and B. J. Sutton (2008) Nat. Rev. Immunol. 8:205.
2. Karagiannis, S. N. et al. (2003) Eur. J. Immunol. 33:1030.
3. Maurer, D. et al. (1996) J. Immunol. 157:607.
4. Galli, S. J. and T. Mindy. (2012) Nat Med. 18:693.

Bioinformatics

• Uniprot:
  • P01854-1()