Recombinant Human Activin AC Heterodimer Protein

Images

 
There are currently no images for Activin AC (4879-AC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Activin AC Heterodimer Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing rhActivin AC at 2 nM and rhActivin RIIB/Fc Chimera dilutions at 0.02-41 nM, the concentration of rhActivin RIIB/Fc Chimera (Catalog # 339-RBB) that produces 50% of the optimal binding response is found to be approximately 0.8-4 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Activin AC protein
Human Activin beta A
(Gly311 - Ser426)
Accession # NP_002183
Human Activin beta C
(Gly237 - Ser352)
Accession # NP_005529
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly311 ( beta A subunit) & Gly237 ( beta C subunit)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.9 kDa ( beta A subunit), 12.5 kDa ( beta C subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23 kDa, reducing conditions
Publications
Read Publications using
4879-AC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Activin AC Heterodimer Protein

  • Activin AC

Background

Activins and inhibins are TGF-beta superfamily proteins that regulate a wide range of processes including mesoderm induction, reproductive system development and function, liver growth and regeneration, wound healing, and inflammation. Activins and inhibins share a variety of beta  subunits, while inhibins also possess a unique alpha subunit. There are four human inhibin beta subunits ( beta A, beta B, beta C, and beta E) and a single inhibin alpha subunit, each of which adopts a cysteine-knot structure (1 - 5). Activin AC is a 23 kDa heterodimer of beta A and beta C subunits (6). Human  beta A consists of a 20 amino acid (aa) signal sequence, a 290 aa propeptide, and a 116 aa mature segment, while beta C consists of an 18 aa signal sequence, a 218 aa propeptide, and a 116 aa mature segment (8, 9). The subunit  beta propeptides are required for subunit folding, dimerization, and secretion (10). Mature human beta A shares 64%, 51%, and 44% aa sequence identity with human beta B, beta C, and beta E, respectively. Sequence identity between human, mouse, and rat is 100% for mature beta A, and 91% - 93% for mature  beta C. The beta A and beta C subunits are both expressed in the liver, pituitary, ovary, testis, and adrenal gland (1, 6, 11). The beta C subunit may dimerize with beta A, beta B or the inhibin alpha  subunit (6, 7). Overexpression of  beta C results in increased production of Activin AC as well as a reduction in beta A expression and activin induced signaling (12, 13). The beta C subunit modulates activin induced effects in a variety of systems by forming intracellular dimers with the beta A subunit and impeding the release of Activins A, AB, and B (2, 12). Activins signal through heterodimeric receptor complexes composed of type I (Activin RIA or RIB) and type II (Activin RIIA or RIIB) transmembrane Ser/Thr kinases.

  1. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
  2. Butler, C.M. et al. (2005) Cytokine Growth Factor Rev. 16:377. 
  3. Abe, Y. et al. (2004) Growth Factors 22:105. 
  4. Vitt, U.A. et al. (2001) Mol. Endocrinol. 15:681. 
  5. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157. 
  6. Mellor S.L. et al. (2000) J. Clin. Endocrinol. Metab. 85:4851. 
  7. Ushiro, Y. et. al. (2006) J. Reprod. Devel. 52:487.
  8. Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.
  9. Hötten, G. et al. (1995) Biochem. Biophys. Res. Commun. 206:608.
  10. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
  11. Gold, E.J. et al. (2004) Mol. Cell. Endocrinol. 222:61.
  12. Mellor, S.L. et al. (2003) Endocrinology 144:4410.
  13. Wada, W. et al. (2004) Am. J. Physiol. Endocrinol. Metab. 287:E247.

Publications for Activin AC (4879-AC)(3)

Reviews for Activin AC (4879-AC) (0)

There are no reviews for Activin AC (4879-AC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Activin AC (4879-AC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Activin AC Products

Blogs on Activin AC

There are no specific blogs for Activin AC, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Activin AC Heterodimer Protein and receive a gift card or discount.

Bioinformatics