Rat IgG, Fab Native Protein

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SDS-Page: Rat IgG, Fab Native Protein [NBP1-97014] - SDS-PAGE of Rat IgG Fab Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IP, PAGE
Concentration
5.73 mg/ml

Order Details

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Rat IgG, Fab Native Protein Summary

Description
A full-length protein corresponding to Rat IgG, Fab.

Source: Serum

Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(ab')2. No reaction was observed against anti-Rat IgG F(c) or anti- Papain.
Protein/Peptide Type
Native Protein
Purity
Ion exchange chromatography

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
5.73 mg/ml
Purity
Ion exchange chromatography

Background

Rat IgG Fab Fragment - secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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