Rabies Virus Antibody (Rab-50) - BSA Free

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF
• Clone: Rab-50
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Rabies Virus Antibody (Rab-50) - BSA Free Summary

• Immunogen: Purified virions of SAD-Vnukova strain followed by Pitman-Moore strain.
• Specificity: Clone Rab 50 shows conformation dependent specificity for the viral envelope glycoprotein. Not known to react with any other organism.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Rabies Virus Antibody (Rab-50) - BSA Free

• Glycoprotein

Background

Rabies virus (Neurotropic lyssavirus) is a member of the Rhabdoviridae family. Rabies is a single stranded, neurotropic, negative sense RNA virus which encodes 5 proteins: a glycoprotein, a nucleoprotein, and three others. The mature virus has a bullet shape, a protein coat, and a lipid envelope. The outer surface of the virus is covered with thumblike glycoprotein projections 5-10 nm long and 3 nm in diameter. The virus averages approximately 780 nm in length. Virus infectivity is destroyed by lipid solvents. Rabies virus is a very successful virus, with a very wide range of hosts. It causes an acute, central nervous system infection, characterized by CNS irritation, followed by paralysis and death. Approximately 50,000 human deaths each year are caused by rabies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rabies Virus Antibody (NB100-63045)(2)

Publications using NB100-63045

• Li, C et al. Generation and characterization of the human neutralizing antibody fragment Fab091 against rabies virus. Acta Pharmacol Sin 32: 329-37. 2001-01-01 [PMID: 21278782]
• Macikova, I et al. Common and different antigenic properties of the rabies virus glycoprotein of strains SAD-Vnukovo and Pitman-Moore. Acta Virol 36: 541-550. 1992-01-01 [PMID: 1284868]