Recombinant Human R3HDML GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human R3HDML GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-253 of Human R3HDML
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MPLLPSTVGLAGLLFWAGQAVNALIMPNATPAPAQPESTAMRLLSGLEVPRYRRKRHISVRDMNALLDYHNHIRASVYPPAANMEYMVWDKRLARAAEAWATQCIWAHGPSQLMRYVGQNLSIHSGQYRSVVDLMKSWSEEKWHYLFPAPRDCNPHCPWRCDGPTCSHYTQMVWASSNRLGCAIHTCSSISVWGNTWHRAAYLVCNYAIKGNWIGESPYKMGKPCSSCPPSYQGSCNSNMCFKGLKSNKFTWF

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: R3HDML
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human R3HDML GST (N-Term) Protein

• Cysteine-rich secretory protein R3HDML
• dJ881L22.3
• MGC129564
• peptidase inhibitor R3HDML
• R3H domain (binds single-stranded nucleic acids) containing-like
• R3H domain containing-like

Background

R3HDML, also known as R3H Domain Containing-Like, is a 29kDa 253 amino acid protein. R3HDML is a secreted protein that belongs to the CRISP family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for R3HDML Recombinant Protein (H00140902-P01). (Showing 1 - 1 of 1 FAQ).

1. Do you have an explanation why the 26kDa protein is running at 52kDa? Are there any indices for dimers?
   • This particular protein is distributed by us for a Taiwanese company called Abnova. Abnova adds a GST tag to their recombinant proteins. This GST tag will add approximately 26kDa in size to the protein it is attached to.

Bioinformatics

• Gene Symbol: R3HDML