pSIVA Apoptosis Detection Flow Cytometry Kit

Images

 
Flow Cytometry: Polarity Sensitive Indicator of Viability Apoptosis Kit [IANBD] [NBP2-29611] - pSIVA-IANBD(TM) + PI shows progressive population staining as cells move from healthy towards death.
Flow Cytometry: Polarity Sensitive Indicator of Viability Apoptosis Kit [IANBD] [NBP2-29611] - pSIVA-IANBD(TM) shows progressive population staining as cells move from healthy towards death.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, Flow-CS
Conjugate
IANBD

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

pSIVA Apoptosis Detection Flow Cytometry Kit Summary

Description
Polarity Sensitive Indicator of Viability (pSIVA (Annexin XII)) is an Annexin based, polarity sensitive probe for the spatiotemporal or kinetic analysis of apoptosis and other forms of cell death. pSIVA (Annexin XII) binding is reversible enabling researchers, for the first time, to detect transient PS exposure which is associated with normal physiological processes as well as with reversible or rescuable apoptosis cell death events. pSIVA (Annexin XII) is conjugated to IANBD, a polarity sensitive dye that fluoresces only when pSIVA is bound to the cell membrane. pSIVA's membrane-bound dependent fluorescence and reversible binding properties are a technological leap for detecting PS exposure and offer additional information on the apoptosis pathway and cell survival compared to Annexin V conjugates. Annexin V binding is nonreversible.
Kit Type
Flow Cytometry Kit

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ul/1 million cells
Application Notes
Please see PDF manual attached for specific usage information. Use in Flow cell surface reported in scientific literature (PMID 24804954)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-29611 in the following application:

Publications
Read Publications using
NBP2-29611 in the following applications:

Reactivity Notes

Human and Mouse reactivity reported in scientific literature (PMID: 24804954)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. 10X Binding Buffer (5 mL or 2 mL)
  2. 10X PBS (20 mL or 5 mL)
  3. pSIVA-IANBD (500 uL or 125 uL)
  4. Propidium Iodide Staining Solution (500 uL or 125 uL)

Notes

pSIVA is protected under patent number: 8,541,549

Alternate Names for pSIVA Apoptosis Detection Flow Cytometry Kit

  • apoptosis kinetics
  • apoptosis kit annexin v
  • apoptosis kit flow cytometry
  • apoptosis kit microscopy
  • apoptosis kit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Supplier Logo

Publications for pSIVA Apoptosis Detection Kit (NBP2-29611)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FLOW, Flow-CS, ICC/IF.


Filter By Application
FLOW
(2)
Flow-CS
(3)
ICC/IF
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-29611 Applications Species
Sommer A, Kordowski F, Buch J et al. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nat Commun. May 10 2016 [PMID: 27161080] (FLOW, Human) FLOW Human
Lew ED, Oh J, Burrola PG et al. Differential TAM receptor-ligand-phospholipid interactions delimit differential TAM bioactivities. Elife. 2014 Oct 24 [PMID: 25265470] (FLOW, Mouse)

Details:
Polarity Sensitive Indicator of Viability Flow Assay Kit used for quantifying apoptosis in adherent cells or supernatant cells from Axl_TAM TKO MEF cultures - cells stained with propidium iodide which bind dead cells and pSIVA which fluoresces when bound to PtdSer (data in Figure 4B).
FLOW Mouse
Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID: 24804954] (Flow-CS, Human, Mouse)

Details:
Fig 4: mouse splenocytes, EL4 mouse lymphoma cells, human PBMC, Jurkat human T cells; Fig 5: Jurkat.
Flow-CS Human, Mouse
Nagaria TS, Williams JL, Leduc C et al. Flavopiridol synergizes with sorafenib to induce cytotoxicity and potentiate antitumorigenic activity in EGFR/HER-2 and mutant RAS/RAF breast cancer model systems. Neoplasia. 2013 Aug [PMID: 23908594] (Flow-CS)

Details:
Flow cytometry (Cell surface): MDA-MB-231 (Fig 4A) and MDA-MB-468 (Fig 4B) adenocarcinoma cells.
Flow-CS
Suhane S, Kanzaki H, Arumugaswami V et al. Mitochondrial NDUFS3 regulates the ROS-mediated onset of metabolic switch in transformed cells. Biol Open. 2013 Mar 15 [PMID: 23519235] (Flow-CS)

Details:
pSIVA-IANBD Flow Kit: Flow (Cell Surface): Fig 1 (HEK293 cells). pSIVA-IANBD was used to determine the basal level of apoptosis in HEK cells.
Flow-CS
Demchenko AP. The change of cellular membranes on apoptosis: fluorescence detection. Exp Oncol. 2012 Oct [PMID: 23070011]

Details:
Live imaging: pSIVA as an important advancement in annexin based methodology.
Ruggiero L, Connor MP, Chen J et al. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Proc Natl Acad Sci U S A. 2012 May 22 [PMID: 22566632] (Mouse)

Details:
Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes.
Mouse
Krajewska M, You Z, Rong J et al. Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity. PLoS One. 2011 [PMID: 21957448] (Mouse)

Details:
IF (mouse primary neuron cultures): Figs 2B, 3. Cells were labeled with pSIVA-IANBD for 15 min, fixed and stained with a MAP2 antibody and DAPI. Cells double-stained with both pSIVA-IANBD and MAP2 (neuronal marker) were identified as degenerating neurons.
Mouse
Graewe S, Rankin KE, Lehmann C et al. Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress. PLoS Pathog. 2011 Sep [PMID: 21909271] (ICC/IF)

Details:
IF (HepG2 cells infected with P. berghei parasites), Fig 1
ICC/IF
Warnes G, Martins S. Real-time flow cytometry for the kinetic analysis of oncosis. Cytometry A. 2011 Mar [PMID: 21254392]

Details:
Live imaging: pSIVA as an assay for real-time detection of apoptosis.
Show All 13 Publications.

Review for pSIVA Apoptosis Detection Kit (NBP2-29611) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-29611:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 06/08/2016
View

Summary

ApplicationFlow Cytometry
Sample TestedCEM T cell line
SpeciesHuman
Lot100313

Blocking

Blocking Detailsnone

Primary Anitbody

Dilution Ratiouse product at 1/50 in HBSS, analyze cells after 5- 30 minutes, keep cells dark and at room temperature, do not fix cells

Details

Detection NotesBD accuri flow, use non irradiated cells as negative control
Fixation Detailsspin cells at 1000 rpm for 2 min, resuspend in HBSS
Permeabilization Detailsnone
Wash Descriptionnone

Comments

Commentsimportant: this flow reagent does not work for microscopy. use microscopy pSIVA kit instead (NBP2-29382)

Product General Protocols

View specific protocols for pSIVA Apoptosis Detection Kit (NBP2-29611): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for pSIVA Apoptosis Detection Kit (NBP2-29611). (Showing 1 - 2 of 2 FAQs).

  1. Our end user is working on tissues (epithelium of imaginal disk from drosophila leg). The tissues are kept growing on a culture medium and they manage to follow apoptosis properly with orange acrylin but not with annexin V because of background. Did you ever test any of your pSIVA apoptosis kits (NBP2-29382, NBP2-29611) on tissue culture instead of cell culture ? Is there a chance the proble will work on such an application ? Would you be able to provide a sample for this application ? As for the other samples, I'm still waiting on customer feedback and I'll keep you posted.
    • Yes, pSIVA has been used in tissue applications as denoted on the publication link. Specifically, I would encourage the research to look at the following publications from the link: 1. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Ruggiero L, MP Connor, J Chen, R Langen, SC Finnemann. PNAS 109:8145-4148 (2012). Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). > The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes. 2. Diurnal photoreceptor outer segment shedding: contributions of the RPE. Finnemann SC, L Ruggiero, MP Connor. Invest Opthalmol Vis Sci 53:4327 (2012). Live Imaging: mouse retina. pSIVA was used quantify the surface exposure of phosphotidylserine on the distal rod photoreceptor outer segments. 3. Characterization of cell cycle modifications induced by electric pulses in human corneal endothelium. Thi MH, Z He, N Campolmi, S Piselli, P Gain, M Peoc'H, JM Dumolllard, S Acquart, O Garraud, G Thuret. Acta Opthalmologica 90:s249 (2012). Live tissue imaging (human cornea organ cultures): pSIVA-IANBD was used to assess cell death following gene electrotransfer into corneal endothelial cells. > 3. Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processess and degeneration. Kim YE, J Chen, JR Chan, R Langen. Nature Methods 7:67-73 (2010a). Real-time live-cell imaging and time-lapse microscopy of apoptosis: Fig 2 (COS-7 cells), Fig 3 (rat neuronal degeneration), Fig 4 (rat axonal degeneration), Fig 5 (rescue of rat neuronal degeneration as visualized by pSIVA. (this was also used in a rat nerve injury tissue model) Please watch the pSIVA webinar on our website for additional information about pSIVA. The webinar is referred to March 25, 2014: Real-Time Tracking of Cell Death Webinar. We would certainly be pleased to offer any of your end user research customers a sample who is willing to provide a short explanation about what they are planning to do, their timeframe, and provide feedback about how it worked for them. For any samples, please specify if you
  2. Could you specify the composition of the binding buffer in the pSIVA-INABD Apoptosis/Viability Flow Kit (Cat No:NBP2-29611), because my binding buffer is often exhausted long time before the pSIVA-IANBD and propidium iodide is used up? I assume that it is a standard buffer with additional CaCl2 (like I am preparing it for micrsocopy time lapse studies with the microcopy kit (Cat No: NBP2-29382)).
    • Thank you for your inquiry about pSIVA and for using our products. Here is the requested recipe: 10X Binding Buffer: 100mM HEPES pH 7.4, 1.4M NaCl, 25mM CaCl2

Other Available Formats

IANBD NBP2-29611
IANBD NBP2-29611

Additional pSIVA Apoptosis Detection Products

Blogs on pSIVA Apoptosis Detection

There are no specific blogs for pSIVA Apoptosis Detection, but you can read our latest blog posts.
Coronavirus Brochure

Contact Information

Product PDFs

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
06/08/2016
Application: Flow
Species: Human