Recombinant Protein G His (C-Term) Protein

SDS-Page: Protein G His (C-Term) Protein [NBP2-35035]

Protein G His (C-Term) Protein [NBP2-35035]

SDS-Page: Protein G His (C-Term) Protein [NBP2-35035]

HPLC: Recombinant Protein G Protein [NBP2-35035] - Protein G His (C-Term) Protein [NBP2-35035]

Product Discontinued

Recombinant Protein G His (C-Term) Protein Summary

• Description: A single, non-glycosylated biologically active polypeptide chain with a 6xHis tag on the C-terminal containing 210 residues of Protein G protein. It migrates with an apparent molecular mass of 29kDa in SDS-PAGE.
  
  

  Source: E. coli

  Amino Acid Sequence: MGTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTK AVDAETAEKAFKQYANDNGV DGVWTYDDAT KTFTVTEKLA AALEHHHHHH

• Details of Functionality: Protein G is a genetically engineered protein containing 3 IgG-binding regions of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein G to ensure the maximum specific IgG binding. The recombinant Protein G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 22.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from solution of 20mM PB (pH 7.0)
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Reconstitute with distilled water or saline.

Alternate Names for Recombinant Protein G His (C-Term) Protein

• IgG binding protein G Immunoglobulin G binding protein G [Precursor] spg

Background

Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.