Protein A Antibody - BSA Free

Western Blot: Protein A Antibody [NBP2-53424] - Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: approx. 50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.

Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A. Lane 2: None. Load: 50 ng per lane. Primary antibody: Protein A antibody at 1:1,000 overnight at 4C. Secondary antibody: IRDye800(R) goat secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 43 kDa, 53 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.

• Reactivity: Ba
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

Protein A Antibody - BSA Free Summary

• Description: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Goat Serum
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Protein A [Staphylococcus aureus]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SAOUHSC_00069
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Immunoprecipitation 1:1000-1:5000
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been tested by western blot and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Optimal concentration in immunoassays should be determined by the researcher.

Reactivity Notes

Cross reactivity against Protein A from other sources is unknown

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Protein A Antibody - BSA Free

• Immunoglobulin G binding protein A
• PROA
• Protein A
• Protein-A
• SPA
• Staphylococcal protein A

Background

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Protein A Antibody (NBP2-53424). (Showing 1 - 1 of 1 FAQ).

1. During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!
   • We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.

Bioinformatics

• Gene Symbol: SAOUHSC_00069