Pma1 Antibody (40B7)

• Reactivity: Ye
• Applications: WB, ICC/IF, IHC
• Clone: 40B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Pma1 Antibody (40B7) Summary

• Immunogen: Generated from a sequence of yeast Pma1 [Swiss-Prot# P05030]
• Localization: Plasma Membrane
• Marker: Plasma Membrane Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: Pma1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry 1:1000-1:5000
  • Western Blot 1:10000
• Application Notes: This Pma1 Antibody (40B7) is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. For Western blots of yeast protein samples (cell lysates), use at a dilution of 1:10,000, followed by chemiluminescent detection (ECL). For non-ECL western detection methods, use at a dilution of 1:1,000 to 1:5,000. The predicted molecular weight by western blot is 100 kDa.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Saccharomyces cerevisiae.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated hybridoma cell culture media.
• Preservative: 0.035% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Pma1 Antibody (40B7)

• Plasma membrane ATPase 1
• PMA1
• Proton pump 1
• YGL008C

Background

Pma1 (plasma membrane H+-ATPase) belongs to cation transport ATPase (P-type) (TC 3.A.3) family and is an essential protein in yeast/Saccharomyces cerevisiae wherein it pumps out H+ for controlling intracellular pH as well as for generating an electrochemical gradient required to drive cellular import of metabolites (this activity utilizes cellular ATP). Glucose acts as an inducer of Pma1 expression and its catalytic activity through phosphorylation at Ser-899, Thr-912 and Ser-911 sites. Upon glucose starvation, Pma1 is negatively regulated by casein kinase I (Yck1p and Yck2p) phosphorylation and glucose mediated Pma1 activation requires glucose sensor Snf3p, G-protein Gpa2p and transport/phosphorylation of glucose. Hsp30 negatively regulate Pma1 activity during heat shock conditions and contributes towards thermo-tolerance by lowering cellular ATP consumption. Pma1 expression has been shown to induce oncogenic transformation of NIH 3T3 cells and cause increased intracellular pH. Pma1 has emerged as a potential target for development of anti-fungal agents, its suitability as a biomarker for toxicity mediated stress and as a model for study of membrane protein biogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pma1 Antibody (NB120-4645)(2)

Publications using NB120-4645

• Kha CX, Nava I, Tseng KA., et Al. V-ATPase Regulates Retinal Progenitor Cell Proliferation During Eye Regrowth in Xenopus J Ocul Pharmacol Ther 2023-03-03 [PMID: 36867156]
• Velivela SD, Kane PM. Compensatory Internalization of Pma1 in V-ATPase Mutants in Saccharomyces cerevisiae Requires Calcium- and Glucose-Sensitive Phosphatases. Genetics 2017-12-18 [PMID: 29254995] (Yeast)

Bioinformatics

• Gene Symbol: Pma1