Plasmacytoid Dendritic Cell Assay Kit

Product Discontinued

Plasmacytoid Dendritic Cell Assay Kit Summary

• Description: Plasmacytoid Dendritic Cell (pDC)/TLR9 Kit is validated by Flow Cytometry for identifying human TLR9 expression in pDC from human whole blood, freshly isolated peripheral blood mononuclear cells (PBMC) and frozen PBMC.
  Before you begin:
  It is recommended that users follow the protocol provided for the best results with this kit. Permeabilization buffer is supplied as a 10X solution. Dilute with deionized water to its fi nal 1X working concentration immediately prior to use (example: 1 ml of 10X Permeabilization buffer to 9 ml of deionized water). Single color (AF647, FITC, PE, PerCP-Cy5.5) stained samples are recommended as compensation controls for fl ow cytometric analysis. Reagents for compensation controls are not provided in this kit. All staining and incubation steps should be done using light protected procedures. Commonly this is done by covering the sample racks or ice bucket with tin foil.
• Kit Type: Assay Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Plasmacytoid Dendritic Cell Assay Kit

• Human Plasmacytoid Dendritic Cell (pDC)
• Human Plasmacytoid Dendritic Cell
• Plasmacytoid Dendritic Cell (pDC)

Background

Plasmacytoid Dendritic Cells (pDC) are a subset of peripheral blood Dendritic Cells which play an important role in both innate and adaptive immune responses. pDC recognize viral components via Toll-like Receptors (TLR) including TLR7 and TLR9, and then rapidly produce Interferon-alpha (IFNalpha) as a primary anti-viral immune response. pDC also participate in antigen presentation, an important functional role which drives the adaptive immune response. Intense investigation has led to pDC identifi cation with a unique set of markers by fl ow cytometry. pDC are usually present in relatively low frequency in peripheral blood. Direct identifi cation of subsets of peripheral blood DCs has an advantage in analyzing changes in number or function of DC subsets during chronic viral infections or diseased patients, as examples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Plasmacytoid Dendritic Cell Kit (NBP2-29610)(1)

Publication using NBP2-29610

• Sato D, Suzuki Y, Kano T et al. Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse therapy in IgA nephropathy patients. Nephrol Dial Transplant. 2012-03-01 [PMID: 21778277] (Flow Cytometry Control, Flow-CS, Human)
  
  Details:
  Plasmacytoid Dendritic Cell (pDC)/TLR9 Kit (IMG-6630K) & Human Lineage Marker/HLA-DR Antibody Mix (hLMAX). Flow (cell surface & intracellular): Primary human tonsillar B cells, Fig 3B.