Plasma Cell Marker Antibody (SPM310) - Azide and BSA Free

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) - Azide and BSA Free [NBP2-34407] - Formalin-fixed paraffin-embedded human tonsil stained with plasma cell marker Monoclonal antibody (SPM310)

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) - Azide and BSA Free [NBP2-34407] - Formalin-paraffin human tonsil stained with Plasma Cell Marker MAb (SPM310).

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) - Azide and BSA Free [NBP2-34407] - Human tonsil tissue. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.

• Reactivity: Hu, Rt(-)
• Applications: IHC
• Clone: SPM310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Plasma Cell Marker Antibody (SPM310) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34359).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Pancreatic cancer-related mucin
• Localization: Cytoplasmic
• Marker: Plasma Cell Marker
• Specificity: It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This monoclonal antibody superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this monoclonal antibody is not suitable for staining frozen tissues.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Note that this antibody is not suitable for staining frozen tissues.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.