![Immunohistochemistry-Paraffin: Plasma Cell Antibody (LIV3G11 (7B18)) [Azide Free] [NBP2-33162] - Formalin-paraffin human tonsil stained with Plasma Cell Marker MAb (LIV3G11).](http://images.novusbio.com/fullsize/Plasma-Cell-Antibody-LIV3G11-7B18-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33162-img0001.jpg "Immunohistochemistry-Paraffin: Plasma Cell Antibody (LIV3G11 (7B18)) [Azide Free] [NBP2-33162] - Formalin-paraffin human tonsil stained with Plasma Cell Marker MAb (LIV3G11).")
| Reactivity | Hu, Rt(-)Species Glossary |
| Applications | IHC |
| Clone | LIV3G11 (7B18) |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-25239). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Pancreatic cancer-related mucin |
| Localization | Cytoplasmic |
| Marker | Plasma Cell Marker |
| Specificity | It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This monoclonal antibody superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this monoclonal antibody is not suitable for staining frozen tissues. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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