PiColorLock™ Phosphate Detection Reagent


PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the ...read more
PiColorLock Gold Colorimetric Assay Kit [303-0125] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate ...read more
PiColorLock Gold Colorimetric Assay Kit [303-0125] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.
PiColorLock Gold Colorimetric Assay Kit [303-0125] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor ...read more

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PiColorLock™ Phosphate Detection Reagent Summary

PiColorLock phosphate detection reagent changes colour in the presence of inorganic phosphate (Pi). This can be exploited to measure any enzyme that generates Pi including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins.

The unique formulation of PiColorLock affords enhanced assay linearity, dynamic range and colour stability; it is also possible with PiColorLock to work with unstable substrates (e.g. ATP, GTP) that give high non-enzymatic background signals with other acidic dye-based detection reagents.

Colorimetric assaySimple, scalable and non-radioactive
Compatible with almost any assay bufferFlexibility over a wide range of existing assays
Stable reagent formulationLong shelf life
Unique accelerator and stabilizerSpeeds up color development
PiColorLock-Pi complex is very stableNo precipitation, results can be measured over several hours - ideal for HTS
Wide linear rangeNo inhibition of color development by high concentrations of protein


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303-0125 in the following applications:

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Store at 4C.


This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.


Phosphate Detection Reagent for Measuring Phosphatases, ATPases and Nucleotidases. Superior Colorimetric System. Unparalleled stability of Pi-dye complexes. Special additive ensures low backgrounds with acid-labile substrates. Reagent is compatible with almost any assay buffer. No inhibition of color development by high concentrations of protein. Stable reagent formulation long shelf life.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for PiColorLock (TM) Phosphate Detection Reagent (303-0125)(82)

We have publications tested in 1 confirmed species: Parasite.

We have publications tested in 1 application: EnzAct.

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Publications using 303-0125 Applications Species
El Gendy MAM, Hassanein H, Saleh FM et al. Hydrazonoyl chlorides possess promising antitumor properties Life sciences 2022-02-07 [PMID: 35143825]
Medellin B, Yang W, Konduri S et al. Targeted Covalent Inhibition of Small CTD Phosphatase 1 to Promote the Degradation of the REST Transcription Factor in Human Cells Journal of medicinal chemistry 2021-12-21 [PMID: 34931516]
Brewer M, Machio-Castello M, Viana R Et al. An empirical pipeline for personalized diagnosis of Lafora disease mutations iScience 2021-11-01 [PMID: 34755096]
Zhou L, Zhang Q, Zhang P et al. c-Abl-mediated Drp1 phosphorylation promotes oxidative stress-induced mitochondrial fragmentation and neuronal cell death. Cell Death Dis. 2017-10-12 [PMID: 29022905]
Basu A, Yap MN. Disassembly of the Staphylococcus aureus hibernating 100S ribosome by an evolutionarily conserved GTPase. Proc Natl Acad Sci U S A. 2017-09-26 [PMID: 28894000]
Kandimalla R, Manczak M, Fry D et al. Reduced Dynamin-related Protein 1 Protects Against Phosphorylated Tau-induced Mitochondrial Dysfunction and Synaptic Damage in Alzheimer’s Disease. Hum Mol Genet. 2016-11-15 [PMID: 28173111]
Press O, Zvagelsky T, Vyazmensky M et al. Construction of Structural Mimetics of the Thyrotropin Receptor Intracellular Domain. Biophys J. 2016-12-20 [PMID: 28002738]
Dores-Silva PR, Nishimura LS, Kiraly VT et al. Structural and functional studies of the Leishmania braziliensismitochondrial Hsp70: Similarities and dissimilarities to human orthologues. Arch Biochem Biophys. 2017-01-01 [PMID: 27840097]
Eniyan K, Kumar A, Rayasam GV et al. Development of a one-pot assay for screening and identification of Mur pathway inhibitors in Mycobacterium tuberculosis. Sci Rep. 2016-10-13 [PMID: 27734910]
Habeck M, Tokhtaeva E, Nadav Y et al. Selective assembly of Na,K-ATPase alpha2beta2 heterodimers in the heart: distinct functional properties and isoform-selective inhibitors. J Biol Chem. 2016-10-28 [PMID: 27624940]
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FAQs for PiColorLock (TM) Phosphate Detection Reagent (303-0125). (Showing 1 - 4 of 4 FAQs).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.

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