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![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0001.jpg "PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0002.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0008.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0007.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0003.jpg "PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0004.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0005.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0006.jpg)