PiColorLock™ Phosphate Detection Reagent


PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the ...read more
PiColorlock Gold Colorimetric Assay Kit [303-0030] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate ...read more
PiColorlock Gold Colorimetric Assay Kit [303-0030] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, ...read more
PiColorlock Gold Colorimetric Assay Kit [303-0030] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.

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PiColorLock™ Phosphate Detection Reagent Summary

PiColorLock phosphate detection reagent changes colour in the presence of inorganic phosphate (Pi). This can be exploited to measure any enzyme that generates Pi including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins.

The unique formulation of PiColorLock affords enhanced assay linearity, dynamic range and colour stability; it is also possible with PiColorLock to work with unstable substrates (e.g. ATP, GTP) that give high non-enzymatic background signals with other acidic dye-based detection reagents.

Colorimetric assaySimple, scalable and non-radioactive
Compatible with almost any assay bufferFlexibility over a wide range of existing assays
Stable reagent formulationLong shelf life
Unique accelerator and stabilizerSpeeds up color development
PiColorLock-Pi complex is very stableNo precipitation, results can be measured over several hours - ideal for HTS
Wide linear rangeNo inhibition of color development by high concentrations of protein


Reviewed Applications
Read 3 Reviews rated 5
303-0030 in the following application:

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303-0030 in the following applications:

Packaging, Storage & Formulations

Store at 4C.


This product is manufactured by Abcam and distributed by Novus Biologicals.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for PiColorLock (TM) Phosphate Detection Reagent (303-0030)(82)

We have publications tested in 1 confirmed species: Parasite.

We have publications tested in 1 application: EnzAct.

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Showing Publications 1 - 10 of 82. Show All 82 Publications.
Publications using 303-0030 Applications Species
Wang R, Dong Y, Lu Y et al. Photobiomodulation for Global Cerebral Ischemia: Targeting Mitochondrial Dynamics and Functions. Mol. Neurobiol. Jun 27 2018 [PMID: 29951942]
Zhou L, Zhang Q, Zhang P et al. c-Abl-mediated Drp1 phosphorylation promotes oxidative stress-induced mitochondrial fragmentation and neuronal cell death. Cell Death Dis. 2017 Oct 12 [PMID: 29022905]
Basu A, Yap MN. Disassembly of the Staphylococcus aureus hibernating 100S ribosome by an evolutionarily conserved GTPase. Proc Natl Acad Sci U S A. 2017 Sep 26 [PMID: 28894000]
Kandimalla R, Manczak M, Fry D et al. Reduced Dynamin-related Protein 1 Protects Against Phosphorylated Tau-induced Mitochondrial Dysfunction and Synaptic Damage in Alzheimer’s Disease. Hum Mol Genet. 2016 Nov 15 [PMID: 28173111]
Press O, Zvagelsky T, Vyazmensky M et al. Construction of Structural Mimetics of the Thyrotropin Receptor Intracellular Domain. Biophys J. 2016 Dec 20 [PMID: 28002738]
Dores-Silva PR, Nishimura LS, Kiraly VT et al. Structural and functional studies of the Leishmania braziliensismitochondrial Hsp70: Similarities and dissimilarities to human orthologues. Arch Biochem Biophys. 2017 Jan 1 [PMID: 27840097]
Eniyan K, Kumar A, Rayasam GV et al. Development of a one-pot assay for screening and identification of Mur pathway inhibitors in Mycobacterium tuberculosis. Sci Rep. 2016 Oct 13 [PMID: 27734910]
Habeck M, Tokhtaeva E, Nadav Y et al. Selective assembly of Na,K-ATPase alpha2beta2 heterodimers in the heart: distinct functional properties and isoform-selective inhibitors. J Biol Chem. 2016 Oct 28 [PMID: 27624940]
Ye Q, Yang Y, van Staalduinen L et al. Structure of the Dictyostelium Myosin-II Heavy Chain Kinase A (MHCK-A) [alpha]-kinase domain apoenzyme reveals a novel autoinhibited conformation. Sci Rep. 2016 May 23 [PMID: 27211275]
Paresi CJ, Liu Q, Li YM. Benzimidazole covalent probes and the gastric H+/K+-ATPase as a model system for protein labeling in a copper-free setting. Mol Biosyst. 2016 [PMID: 26952080]
Show All 82 Publications.

Reviews for PiColorLock (TM) Phosphate Detection Reagent (303-0030) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 review tested in 1 species: Other.

Reviews using 303-0030:
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reviewed by:
David White
ICC/IF 01/22/2019


reviewed by:
David White


ELISA PiColorLock (TM) Phosphate Detection Reagent 303-0030
reviewed by:
Hilary Frase
ELISA Other 06/08/2012


Sample Testedpurified recombinant enzyme
CommentsI used this product to measure Pi release by a recombinant enzyme. I was able to do this in a 96-well plate format and even decreased the reagent used to 1/2 when I used 1/2 well plates. I was extremely pleased with the linearity of the reagent to Pi concentration and the long stability of the signal!


Blocking Detailsnone

Primary Anitbody

Dilution Ration/a


Detection NotesPi ColorLock Gold
ELISA Plate Detailsuncoated
Wash Descriptionnone


CommentsI used this product to measure Pi release by a recombinant enzyme. I was able to do this in a 96-well plate format and even decreased the reagent used to 1/2 when I used 1/2 well plates. I was extremely pleased with the linearity of the reagent to Pi concentration and the long stability of the signal!

FAQs for PiColorLock (TM) Phosphate Detection Reagent (303-0030). (Showing 1 - 4 of 4 FAQs).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.

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David White
Application: ICC/IF

David White

Hilary Frase
Application: ELISA
Species: Other