Phospho-Tyrosine Antibody (21) - Azide and BSA Free

Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911] - Analysis of Un-treated (-) and treated (+), 1mM Pervanadate treatment for 30min. Raji whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Phosphotyrosine antibody.

Immunocytochemistry/Immunofluorescence: Phospho-Tyrosine Antibody (21) [NBP2-42911] - Analysis of Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Phosphotyrosine protein stained by Phosphotyrosine antibody [0021] diluted at 1:50. Red: paxillin, a cytoskeleton marker, stained by Paxillin antibody diluted at 1:100.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Phospho-Tyrosine Antibody (21) - Azide and BSA Free Summary

• Immunogen: The immunogen used to generate this antibody corresponds to Phospho-Tyrosine
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:1000
  • Western Blot 1:5000-1:20000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Phospho-Tyrosine Antibody (21) - Azide and BSA Free

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• pTyrosine
• pY20
• Tyrosine

Background

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.