Western Blot: Phospho-Tyrosine Antibody (13F9) [NB600-907] - Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coommassie staining. Load: 10 ug per ...read more
ELISA: Phospho-Tyrosine Antibody (13F9) [NB600-907] - ELISA results of Mab anti-phosphotyrosine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1ug of conjugate. The starting ...read more
This product is unpurified. The exact concentration of antibody is not quantifiable.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.01% Sodium Azide
Western Blot 1:500-1:2000
This monoclonal antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated - carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
Read 1 Review rated 5 using NB600-907 in the following applications:
Alternate Names for Phospho-Tyrosine Antibody (13F9)
2 amino 3(4 hydroxyphenyl) propanoic acid
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Reviews for Phospho-Tyrosine Antibody (NB600-907) (1)