PGE1 ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
4.88 - 5,000 pg/ml |
| Sensitivity |
5.58 pg/ml |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
Mean 8.8% |
| Intra-Assay |
Mean 9.3% |
| Spike Recovery |
Tissue Culture Media - 90-110% Human Saliva - 107.2% Human Urine - 109.9% Human Serum - 87.0% Human Plasma - 107.7% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Antibody
- Assay buffer
- Conjugate
- DxS IgG Microtiter plate
- pNpp Substrate
- Standard
- Stop solution
- Wash buffer concentrate
|
Alternate Names for PGE1 ELISA Kit (Colorimetric)
Background
Prostaglandin E1 (PGE1) is synthesized from dihomo-Y-linolenic acid (DGLA). PGE1 has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are induced by receptor mediated elevation of cAMP. PGE1 is the major prostaglandin in semen.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for PGE1 ELISA Kit (NBP2-61296). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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