PGBD2 Antibody

• Reactivity: Hu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PGBD2 Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence C-ESNADTTSQGRRSRR corresponding to internal region according to NP_001017434.1, NP_733843.1 .
• Epitope: C-ESNADTTSQGRRSRR
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001017434.1; NP_733843.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PGBD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave an approx. 30 kDa band in human brain (cerebral cortex) lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 39.6 kDa band according to NP_001017434.1 and of 68.0 kDa band according to NP_733843.1. The 30 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGBD2 Antibody

• piggyBac transposable element derived 2
• piggyBac transposable element-derived protein 2

Background

The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. The exact function of this gene is not known. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGBD2 Antibody (NB100-93427)(1)

Publication using NB100-93427

• Wu SC, Meir YJ, Coates CJ et al. piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells. Proc Natl Acad Sci U S A 2006-10-10 [PMID: 17005721]

Bioinformatics

• Gene Symbol: PGBD2
• Entrez:
  • 267002(Human)
• Uniprot:
  • NP_001017434.1(Human)