Peroxidase Antibody

• Reactivity: All-NA
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Peroxidase Antibody Summary

• Description: Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.
• Immunogen: Peroxidase (Horseradish)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoprecipitation, and most immunological methods requiring high titer and specificity.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Peroxidase Antibody

• EC 1.11.1.7
• Horseradish Peroxidase
• HPRC 1
• HPRC1
• HRPA2
• HRPC 2
• HRPC2
• HRPE5
• HRPN
• Neutral peroxidase
• Peroxidase A2
• Peroxidase C1A
• Peroxidase C1B
• Peroxidase C2
• Peroxidase C3
• Peroxidase E5
• Peroxidase N
• PRXC1A
• PRXC1B
• PRXC2
• PRXC3

Background

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.