pCL-Ampho Retrovirus Packaging Vector

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Product Details

Summary
Reactivity Ma, Ha(-)Species Glossary
Applications RetVir
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

pCL-Ampho Retrovirus Packaging Vector Summary

Specificity
Ectotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications/Dilutions

Application Notes
For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin. Use in retroviral production reported in scientific literature (PMID: 24311986)
Publications
Read Publications using
NBP2-29541 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24311986)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 20 ul sterile water.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

Sequence information for this plasmid is not available. This packaging vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual for additional information.

Alternate Names for pCL-Ampho Retrovirus Packaging Vector

  • pcl-ampho packaging vector
  • pCL-Ampho Retrovirus
  • pCLAmpho
  • pCL-Ampho

Background

The pCL-Ampho packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of 1 infectious particle/cell/day. Retrovirus tropism is determined at three levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. The plasmid DNA can be amplified by transforming bacteria and growing them on LB/ampicillin (100 ug/ml) media.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for pCL-Ampho Retrovirus Packaging Vector (NBP2-29541)(38)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: Ret Vir, RetVir.


Filter By Application
Ret Vir
(2)
RetVir
(11)
All Applications
Filter By Species
Human
(9)
Rat
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NBP2-29541 Applications Species
Zingg D, Debbache J, Pena-Hernandez R et al. EZH2-Mediated Primary Cilium Deconstruction Drives Metastatic Melanoma Formation. Cancer Cell. Jul 9 2018 [PMID: 30008323] (RetVir) RetVir
Plenker D. Functional analysis of CD74-NRG1-a new recurrent oncogenic gene fusion in lung adenocarcinoma Thesis. 2015 (RetVir) RetVir
Di Mattia T, Wilhelm LP, Ikhlef S et al. Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites. EMBO Rep. Jun 1 2018 [PMID: 29858488] (RetVir) RetVir
Wiggins BG. Investigation of the role of atypical chemokine receptors CCRL1 and CCRL2 in antibody aesponses and the battle of the CARs: CD28 costimulatory endodomains endow T Cells with more robust effector functions in vitro compared to their 4-1BB counterparts Thesis. 2014 (RetVir) RetVir
Arshad N, Cresswell P. Tumor-associated calreticulin variants functionally compromise the peptide loading complex and impair its recruitment of MHC-I J. Biol. Chem. May 16 2018 [PMID: 29769311] (RetVir) RetVir
Zhang Z, Wu WS. Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming. Methods Mol. Biol. 2018 Feb 14 [PMID: 29435939] (Human) Human
Wong VC, Bass VL, Bullock ME et al. NF-kB-Chromatin Interactions Drive Diverse Phenotypes by Modulating Transcriptional Noise Cell Rep 2018 Jan 16 [PMID: 29346759] (RetVir) RetVir
Hu C, Yu M, Ren Y et al. PP2A inhibition from LB100 therapy enhances daunorubicin cytotoxicity in secondary acute myeloid leukemia via miR-181b-1 upregulation. Sci Rep. 2017 Jun 06 [PMID: 28588271]
Wilhelm LP, Wendling C, Vedie B et al. STARD3 mediates endoplasmic reticulum-to-endosome cholesterol transport at membrane contact sites. EMBO J. Apr 4 2017 [PMID: 28377464]
Fu B, Wang L, Li S, Dorf ME. ZMPSTE24 defends against influenza and other pathogenic viruses. J. Exp. Med. Feb 28 2017 [PMID: 28246125]
Show All 38 Publications.

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Product General Protocols

View specific protocols for pCL-Ampho Retrovirus Packaging Vector (NBP2-29541): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for pCL-Ampho Retrovirus Packaging Vector (NBP2-29541). (Showing 1 - 3 of 3 FAQs).

  1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.
    • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
  2. We would like to make sure that your pCL-Ampho Retrovirus Packaging Vector is compatible with the MSCV (Murine Stem Cell Virus) Retroviral Expression System.
    • The pCL-Ampho Retrovirus Packaging Vector NBP2-29541 should be compatible with a MSCV expression system. Please see the following publication that utilized MSCV2.2 with this packaging vector: Publication link
  3. I was curious if you had a map for the pcl-ampho vector?
    • Unfortunately, we do not have a pCL-Ampho Vector Map in our records. This product was originally developed by Imgenex, a company we acquired at the beginning of last year, and it does not appear that the vector maps were supplied to us. We do have several publications referencing this product and you may find some of those publications useful: click on Reviews & Publications tab at NBP2-29541.

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