PAR/pADPr Antibody (10HA) [Alexa Fluor® 350]

• Reactivity: All-Multi
• Applications: ELISA, IHC, IP, AP, Flow, ICC/IF
• Clone: 10HA
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

PAR/pADPr Antibody (10HA) [Alexa Fluor® 350] Summary

• Immunogen: Purified ADP-ribose polymers between 2 and 50 units long
• Specificity: The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers. Detects Poly(ADP-ribose) Polymer in Direct ELISA.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Intracellular Staining by Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAR/pADPr Antibody (10HA) [Alexa Fluor® 350]

• pADPr
• PAR
• Poly(ADP-ribose)

Background

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.