PAR/pADPr Antibody (10HA) [Unconjugated]

Jurkat human acute T cell leukemia cell line treated with 1 mM H2O2 for 5 minutes (filled histogram) or resting (open histogram) were stained with Mouse Anti-PAR Monoclonal Antibody (Catalog # 4335-MC-100) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

PAR/pADPr was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

PAR/pADPr was detected in immersion fixed 786‑O human renal cell adenocarcinoma cell line untreated (negative) or treated (positive) with 2mM hydrogen peroxide for 5 minutes using Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus upon stimulation with hydrogen peroxide. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: All-Multi
• Applications: ELISA, IHC, IP, AP, Flow, ICC/IF
• Clone: 10HA
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PAR/pADPr Antibody (10HA) [Unconjugated] Summary

• Immunogen: Purified ADP-ribose polymers between 2 and 50 units long
• Specificity: The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers. Detects Poly(ADP-ribose) Polymer in Direct ELISA.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as 100 μL of a 0.2 μm filtered solution in TBS, at a concentration of 1 mg/mL

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAR/pADPr Antibody (10HA) [Unconjugated]

• pADPr
• PAR
• Poly(ADP-ribose)

Background

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAR/pADPr Antibody (4335-MC-100)(65)

Publications using 4335-MC-100

• Yang, C;Qu, J;Cheng, Y;Tian, M;Wang, Z;Wang, X;Li, X;Zhou, S;Zhao, B;Guo, Y;Zheng, L;Tong, Q; YY1 drives PARP1 expression essential for PARylation of NONO in mRNA maturation during neuroblastoma progression Journal of translational medicine 2024-12-27 [PMID: 39731187] (Western Blot, Human)
• Alirzayeva, H;Loureiro, R;Koyuncu, S;Hommen, F;Nabawi, Y;Zhang, WH;Dao, TTP;Wehrmann, M;Lee, HJ;Vilchez, D; ALS-FUS mutations cause abnormal PARylation and histone H1.2 interaction, leading to pathological changes Cell reports 2024-08-27 [PMID: 39167487] (Immunoprecipitation, Human)
• Chudy, P;Kochan, J;Wawro, M;Nguyen, P;Gorczyca, M;Varanko, A;Retka, A;Ghadei, SS;Napieralska, E;Grochot-Prz?czek, A;Szade, K;Berendes, LS;Park, J;Soko?owski, G;Yu, Q;Józkowicz, A;Nowak, WN;Krzeptowski, W; Heme oxygenase-1 protects cells from replication stress Redox biology 2024-06-19 [PMID: 39047636] (Western Blot, N/A)
• Shu, Y;Jin, X;Ji, M;Zhang, Z;Wang, X;Liang, H;Lu, S;Dong, S;Lin, Y;Guo, Y;Zhuang, Q;Wang, Y;Lei, Z;Guo, L;Meng, X;Zhou, G;Zhang, W;Chang, L; Ku70 Binding to YAP Alters PARP1 Ubiquitination to Regulate Genome Stability and Tumorigenesis Cancer research 2024-06-11 [PMID: 38862269] (Immunocytochemistry, Mouse)
• Saha, S;Huang, SN;Yang, X;Saha, LK;Sun, Y;Khandagale, P;Jenkins, LM;Pommier, Y; The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes Nature communications 2023-11-18 [PMID: 37980342] (Westen Blot, Human)
• Wu, AY;Sekar, P;Huang, DY;Hsu, SH;Chan, CM;Lin, WW; Spatiotemporal roles of AMPK in PARP-1- and autophagy-dependent retinal pigment epithelial cell death caused by UVA Journal of biomedical science 2023-11-07 [PMID: 37936170] (Human)
• Maestri, D;Napoletani, G;Kossenkov, A;Preston-Alp, S;Caruso, LB;Tempera, I; The three-dimensional structure of the EBV genome plays a crucial role in regulating viral gene expression in EBVaGC Nucleic acids research 2023-10-27 [PMID: 37889078] (Western Blot, Human)
• Li, P;Zhen, Y;Kim, C;Liu, Z;Hao, J;Deng, H;Deng, H;Zhou, M;Wang, XD;Qin, T;Yu, Y; Nimbolide targets RNF114 to induce the trapping of PARP1 and synthetic lethality in BRCA-mutated cancer Science advances 2023-10-27 [PMID: 37878693] (Western Blot, Human)
• Li, J;Liu, X;Peng, B;Feng, T;Zhou, W;Meng, L;Zhao, S;Zheng, X;Wu, C;Wu, S;Chen, X;Xu, X;Sun, J;Li, J; O-GlcNAc has crosstalk with ADP-ribosylation via PARG The Journal of biological chemistry 2023-10-17 [PMID: 37858678] (Western Blot, Human)
• Zhu, Y;Zhang, JL;Yan, XJ;Ji, Y;Wang, FF; Exploring a new mechanism between lactate and VSMC calcification: PARP1/POLG/UCP2 signaling pathway and imbalance of mitochondrial homeostasis Cell death & disease 2023-09-07 [PMID: 37679327] (Western Blot, Rat)
• Chen, W;E, Q;Sun, B;Zhang, P;Li, N;Fei, S;Wang, Y;Liu, S;Liu, X;Zhang, X; PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activation Cell death & disease 8/15/2023 [PMID: 37582914]
• Minwoo Wie, Keon Woo Khim, Arnold S Groehler IV, Soomin Heo, Junhyeok Woo, Kook Son et al. Alkylation of nucleobases by 2-chloro-N,N-diethylethanamine hydrochloride (CDEAH) sensitizes PARP1-deficient tumors NAR Cancer 2023-08-07 [PMID: 37554969]
• Yang, S;Hwang, S;Kim, B;Shin, S;Kim, M;Jeong, SM; Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation Cell death & disease 2023-07-15 [PMID: 37454129] (Western Blot, Human)
• Louise Ramos, Sarah Truong, Beibei Zhai, Jay Joshi, Fariba Ghaidi, Michael M. Lizardo et al. A bi-functional PARP-HDAC inhibitor with activity in Ewing sarcoma Clinical Cancer Research 2023-09-01 [PMID: 37279093]
• C Han, B McNamara, S Bellone, J Harold, P Manara, TMP Hartwich, L Mutlu, Y Yang-Hartw, M Zipponi, C Demirkiran, MS Verzosa, G Altwerger, E Ratner, GS Huang, M Clark, V Andikyan, M Azodi, PR Dottino, PE Schwartz, AD Santin The Poly (ADP-ribose) polymerase inhibitor olaparib and pan-ErbB inhibitor neratinib are highly synergistic in HER2 overexpressing epithelial ovarian carcinoma in vitro and in vivo Gynecologic oncology, 2023-01-25;170(0):172-178. 2023-01-25 [PMID: 36706643] (Western Blot, Human)
• H Qi, RH Grace Wrig, M Beato, BD Price The ADP-ribose hydrolase NUDT5 is important for DNA repair Cell Reports, 2022-12-20;41(12):111866. 2022-12-20 [PMID: 36543120] (ICC, Human)
• L Wang, D Wang, O Sonzogni, S Ke, Q Wang, A Thavamani, F Batalini, SA Stopka, MS Regan, S Vandal, S Tian, J Pinto, AM Cyr, VC Bret-Moune, G Baquer, HP Eikesdal, M Yuan, JM Asara, YJ Heng, P Bai, NYR Agar, GM Wulf PARP-inhibition reprograms macrophages toward an anti-tumor phenotype Cell Reports, 2022-10-11;41(2):111462. 2022-10-11 [PMID: 36223740] (Western Blot, Mouse, Transgenic Mouse)
• L Abdelghany, T Kawabata, S Goto, K Jingu, TS Li Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells Translational Oncology, 2022-10-04;26(0):101548. 2022-10-04 [PMID: 36206675] (Western Blot, Human)
• O Deng, S Dash, TC Nepomuceno, B Fang, SY Yun, EA Welsh, HR Lawrence, D Marchion, JM Koomen, AN Monteiro, U Rix Integrated proteomics identifies PARP inhibitor-induced pro-survival signaling changes as potential vulnerabilities in ovarian cancer The Journal of Biological Chemistry, 2022-09-29;0(0):102550. 2022-09-29 [PMID: 36183837] (Western Blot, Human)
• T Thakar, A Dhoonmoon, J Straka, EM Schleicher, CM Nicolae, GL Moldovan Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling Nature Communications, 2022-09-09;13(1):5323. 2022-09-09 [PMID: 36085347] (Proximity Ligation Assay, Human)
• M Cahuzac, B Péant, AM Mes-Masson, F Saad Development of Olaparib-Resistance Prostate Cancer Cell Lines to Identify Mechanisms Associated with Acquired Resistance Cancers, 2022-08-11;14(16):. 2022-08-11 [PMID: 36010871] (Western Blot, Human)
• W Wong, ED Crane, H Zhang, J Li, TA Day, AE Green, KJ Menzies, JD Crane Pgc-1alpha controls epidermal stem cell fate and skin repair by sustaining NAD+ homeostasis during aging Molecular Metabolism, 2022-08-17;65(0):101575. 2022-08-17 [PMID: 35987498] (Western Blot, Transgenic Mouse)
• C Blessing, K Apelt, D van den He, C Gonzalez-L, MB Rother, M van der Wo, R González-P, A Yifrach, A Parnas, RG Shah, TT Kuo, DEC Boer, J Cai, A Kragten, HS Kim, OD Schärer, ACO Vertegaal, GM Shah, S Adar, H Lans, H van Attiku, AG Ladurner, MS Luijsterbu XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair Nature Communications, 2022-08-13;13(1):4762. 2022-08-13 [PMID: 35963869] (ICC, Human)
• S Zhao, Y Hong, YY Liang, XL Li, JC Shen, CC Sun, LL Chu, J Hu, H Wang, DX Xu, SC Zhang, DD Xu, T Xu, LL Zhao Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast Redox Biology, 2022-07-20;55(0):102414. 2022-07-20 [PMID: 35926314] (Western Blot, Human, Mouse)
• Y Zhang, M Fang, S Li, H Xu, J Ren, L Tu, B Zuo, W Yao, G Liang BTApep-TAT peptide inhibits ADP-ribosylation of BORIS to induce DNA damage in cancer Molecular Cancer, 2022-08-02;21(1):158. 2022-08-02 [PMID: 35918747] (Co-Immunoprecipitation, Human)
• F Trulsson, V Akimov, M Robu, N van Overbe, DAP Berrocal, RG Shah, J Cox, GM Shah, B Blagoev, ACO Vertegaal Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates Nature Communications, 2022-05-18;13(1):2736. 2022-05-18 [PMID: 35585066] (Western Blot, Human)
• JF Linares, T Cid-Diaz, A Duran, M Osrodek, A Martinez-O, M Reina-Camp, HH Kuo, O Elemento, ML Martin, T Cordes, TC Thompson, CM Metallo, J Moscat, MT Diaz-Meco The lactate-NAD+ axis activates cancer-associated fibroblasts by downregulating p62 Cell Reports, 2022-05-10;39(6):110792. 2022-05-10 [PMID: 35545049] (Western Blot, Human)
• L Liu, JJ Sandow, DM Leslie Ped, AL Samson, N Silke, T Kratina, RL Ambrose, M Doerflinge, Z Hu, E Morrish, D Chau, AJ Kueh, C Fitzibbon, M Pellegrini, JS Pearson, MO Hottiger, AI Webb, N Lalaoui, J Silke Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death Science Advances, 2022-05-11;8(19):eabh2332. 2022-05-11 [PMID: 35544574] (Immunoprecipitation, Mouse)
• Xiaohui Lin, Wenxia Jiang, Johannes Rudolph, Brian J Lee, Karolin Luger, Shan Zha PARP inhibitors trap PARP2 and alter the mode of recruitment of PARP2 at DNA damage sites Nucleic Acids Research 2022-04-22 [PMID: 35349716]
• Maxime Cahuzac, Patricia Langlois, Benjamin Péant, Hubert Fleury, Anne-Marie Mes-Masson, Fred Saad Pre-activation of autophagy impacts response to olaparib in prostate cancer cells Communications Biology 2022-03-22 [PMID: 35318456]
• Xiaojing Hou, Changming Cai, Yuanlin He, Shiyu An, Shuqin Zhao, Hao Sun et al. Protective Effect of Minocycline Hydrochloride on the Mouse Embryonic Development Against Suboptimal Environment Frontiers in Cell and Developmental Biology 2022-02-01 [PMID: 35178387]
• Q Chen, K Ma, X Liu, SH Chen, P Li, Y Yu, AKL Leung, X Yu Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis Cell Discovery, 2022-01-18;8(1):3. 2022-01-18 [PMID: 35039483] (Immunoprecipitation, Western Blot, Human)
• C Sethy, CN Kundu PARP inhibitor BMN-673 induced apoptosis by trapping PARP-1 and inhibiting base excision repair via modulation of pol-beta in chromatin of breast cancer cells Toxicology and Applied Pharmacology, 2022-01-05;436(0):115860. 2022-01-05 [PMID: 34998856] (Western Blot, Human)
• Z Sarwar, N Nabi, SA Bhat, SQ Gillani, I Reshi, M Un Nisa, G Adelmant, J Marto, S Andrabi Interaction of DBC1 with polyoma small T antigen promotes its degradation and negatively regulates tumorigenesis The Journal of Biological Chemistry, 2021-12-16;0(0):101496. 2021-12-16 [PMID: 34921839] (Flow Cytometry, ICC, Immunoprecipitation, Western Blot, Human)
• KL Cooper, LB Volk, DR Dominguez, AD Duran, KJ Ke Jian Li, LG Hudson Contribution of NADPH oxidase to the retention of UVR-induced DNA damage by arsenic Toxicology and Applied Pharmacology, 2021-11-16;434(0):115799. 2021-11-16 [PMID: 34798142] (ELISA Capture, Human)
• SH Park, Y Kim, JS Ra, MW Wie, MS Kang, S Kang, K Myung, KY Lee Timely termination of repair DNA synthesis by ATAD5 is important in oxidative DNA damage-induced single-strand break repair Nucleic Acids Research, 2021-11-18;0(0):. 2021-11-18 [PMID: 34718749] (ICC, Human)
• C Tromans-Co, A Sanchi, GK Moeller, G Timinszky, M Lopes, I Ahel TARG1 protects against toxic DNA ADP-ribosylation Nucleic Acids Research, 2021-10-11;0(0):. 2021-10-11 [PMID: 34508355] (Western Blot, Human)
• Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation Nature Communications, 2021-08-18;12(1):5010. 2021-08-18 [PMID: 34408146] (Western Blot, Human)
• B Peng, R Shi, J Bian, Y Li, P Wang, H Wang, J Liao, WG Zhu, X Xu PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair Nucleic Acids Research, 2021-07-21;49(13):7554-7570. 2021-07-21 [PMID: 34197606] (Immunoprecipitation, Human)
• S Oka, J Leon, K Sakumi, N Abolhassan, Z Sheng, D Tsuchimoto, FM LaFerla, Y Nakabeppu MTH1 and OGG1 maintain a low level of 8-oxoguanine in Alzheimer's brain, and prevent the progression of Alzheimer's pathogenesis Scientific Reports, 2021-03-23;11(1):5819. 2021-03-23 [PMID: 33758207] (IHC, Western Blot, Mouse)
• JM Martí, A Garcia-Dia, D Delgado-Be, F O'Valle, A González-F, O Carlevaris, JM Rodríguez-, JC Amé, F Dantzer, GL King, K Dziedzic, E Berra, E de Álava, AT Amaral, EM Hammond, FJ Oliver Selective modulation by PARP-1 of HIF-1&alpha-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions Redox Biology, 2021-02-01;41(0):101885. 2021-02-01 [PMID: 33581682] (Western Blot, Human)
• WC Chung, S Lee, Y Kim, JB Seo, MJ Song Kaposi's sarcoma-associated herpesvirus processivity factor (PF-8) recruits cellular E3 ubiquitin ligase CHFR to promote PARP1 degradation and lytic replication PloS Pathogens, 2021-01-28;17(1):e1009261. 2021-01-28 [PMID: 33508027] (Western Blot, Human)
• F Matteini, O Andresini, S Petrai, C Battistell, MN Rossi, R Maione Poly(ADP-ribose) Polymerase 1 (PARP1) restrains MyoD-dependent gene expression during muscle differentiation Sci Rep, 2020-09-15;10(1):15086. 2020-09-15 [PMID: 32934320] (Immunoprecipitation, Mouse)
• AE Gibson, C Yeung, SH Issaq, VJ Collins, M Gouzoulis, Y Zhang, J Ji, A Mendoza, CM Heske Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma Oncogenesis, 2020-09-10;9(9):80. 2020-09-10 [PMID: 32908120] (Functional Assay, Human)
• Z Shao, BJ Lee, É Rouleau-Tu, MF Langelier, X Lin, VM Estes, JM Pascal, S Zha Clinical PARP inhibitors do not abrogate PARP1 exchange at DNA damage sites in vivo Nucleic Acids Res., 2020-09-25;0(0):. 2020-09-25 [PMID: 32890402] (Western Blot, Human)
• PL Patidar, T Viera, JC Morales, N Singh, EA Motea, M Khandelwal, FJ Fattah XRN2 interactome reveals its synthetic lethal relationship with PARP1 inhibition Sci Rep, 2020-08-28;10(1):14253. 2020-08-28 [PMID: 32859985] (ICC, Western Blot, Human)
• H Hanzlikova, E Prokhorova, K Krejcikova, Z Cihlarova, I Kalasova, J Kubovciak, J Sachova, R Hailstone, J Brazina, S Ghosh, S Cirak, JG Gleeson, I Ahel, KW Caldecott Pathogenic ARH3 mutations result in ADP-ribose chromatin scars during DNA strand break repair Nat Commun, 2020-07-07;11(1):3391. 2020-07-07 [PMID: 32636369]
• K Mao, J Chen, H Yu, H Li, Y Ren, X Wu, Y Wen, F Zou, W Li Poly (ADP-ribose) polymerase 1 inhibition prevents neurodegeneration and promotes alpha-synuclein degradation via transcription factor EB-dependent autophagy in mutant alpha-synucleinA53T model of Parkinson's disease Aging Cell, 2020-05-31;0(0):e13163. 2020-05-31 [PMID: 32475059] (IHC, Mouse)
• J Calahorra, E Martínez-L, JM Granadino-, JM Martí, A Cañuelo, S Blanco, FJ Oliver, E Siles Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells Sci Rep, 2020-04-14;10(1):6361. 2020-04-14 [PMID: 32286485] (Human)
• G Yang, Y Chen, J Wu, SH Chen, X Liu, AK Singh, X Yu Poly(ADP-ribosyl)ation mediates early phase histone eviction at DNA lesions Nucleic Acids Res., 2020-04-06;0(0):. 2020-04-06 [PMID: 31965183] (Immunoprecipitation, Human)
• L Zhang, DQ Li MORC2 regulates DNA damage response through a PARP1-dependent pathway Nucleic Acids Res., 2019-09-19;47(16):8502-8520. 2019-09-19 [PMID: 31616951] (Western Blot, Human)
• N Li, Y Wang, S Neri, Y Zhen, LWR Fong, Y Qiao, X Li, Z Chen, C Stephan, W Deng, R Ye, W Jiang, S Zhang, Y Yu, MC Hung, J Chen, SH Lin Tankyrase disrupts metabolic homeostasis and promotes tumorigenesis by inhibiting LKB1-AMPK signalling Nat Commun, 2019-09-25;10(1):4363. 2019-09-25 [PMID: 31554794] (Western Blot, Human)
• Y Chen, H Zhang, Z Xu, H Tang, A Geng, B Cai, T Su, J Shi, C Jiang, X Tian, A Seluanov, J Huang, X Wan, Y Jiang, V Gorbunova, Z Mao A PARP1-BRG1-SIRT1 axis promotes HR repair by reducing nucleosome density at DNA damage sites Nucleic Acids Res., 2019-09-19;0(0):. 2019-09-19 [PMID: 31291457] (Western Blot, Human)
• A Jain, LC Agostini, GA McCarthy, SN Chand, A Ramirez, A Nevler, J Cozzitorto, CW Schultz, CY Lowder, KM Smith, ID Waddell, M Raitses-Gu, C Stossel, YG Gorman, D Atias, CJ Yeo, JM Winter, KP Olive, T Golan, MJ Pishvaian, D Ogilvie, DI James, AM Jordan, JR Brody Poly (ADP) ribose glycohydrolase can be effectively targeted in pancreatic cancer Cancer Res., 2019-07-04;0(0):. 2019-07-04 [PMID: 31273064] (Western Blot, Human)
• L Marcar, K Bardhan, L Gheorghiu, P Dinkelborg, H Pfäffle, Q Liu, M Wang, Z Piotrowska, LV Sequist, K Borgmann, JE Settleman, JA Engelman, AN Hata, H Willers Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity Cell Rep, 2019-06-18;27(12):3422-3432.e4. 2019-06-18 [PMID: 31216465] (Western Blot, Human)
• LC Echeverri, MA Ghonim, J Wang, AA Al-Khami, D Wyczechows, HH Luu, H Kim, MD Sanchez-Pi, J Yélamos, LM Yassin, AH Boulares PARP-1 Is Critical for Recruitment of Dendritic Cells to the Lung in a Mouse Model of Asthma but Dispensable for Their Differentiation and Function Mediators Inflamm., 2019-04-24;2019(0):1656484. 2019-04-24 [PMID: 31178661] (Western Blot, Mouse)
• ME Grunewald, Y Chen, C Kuny, T Maejima, R Lease, D Ferraris, M Aikawa, CS Sullivan, S Perlman, AR Fehr The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression PLoS Pathog., 2019-05-16;15(5):e1007756. 2019-05-16 [PMID: 31095648] (Western Blot, Human)
• AP Lombard, C Liu, CM Armstrong, LS D'Abronzo, W Lou, H Chen, M Dall'Era, PM Ghosh, CP Evans, AC Gao Overexpressed ABCB1 Induces Olaparib-Taxane Cross-Resistance in Advanced Prostate Cancer Transl Oncol, 2019-05-07;12(7):871-878. 2019-05-07 [PMID: 31075528] (Western Blot, Human)
• C Wang, W Xu, J An, M Liang, Y Li, F Zhang, Q Tong, K Huang Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2 Nat Commun, 2019-03-13;10(1):1203. 2019-03-13 [PMID: 30867423] (IHC-P, Rat)
• C Bian, C Zhang, T Luo, A Vyas, SH Chen, C Liu, MA Kassab, Y Yang, M Kong, X Yu NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression Nat Commun, 2019-02-11;10(1):693. 2019-02-11 [PMID: 30741937] (ICC, Western Blot, Human)
• M Li, Y Tang, Q Li, M Xiao, Y Yang, Y Wang Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2 Oncogene, 2019-01-16;0(0):. 2019-01-16 [PMID: 30651599] (ChIP, Human)
• Kulkarni A, Oza J, Yao M, Sohail H, Ginjala V, Tomas-Loba A, Horejsi Z, Tan A, Boulton S, Ganesan S Tripartite Motif-containing 33 (TRIM33) protein functions in the poly(ADP-ribose) polymerase (PARP)-dependent DNA damage response through interaction with Amplified in Liver Cancer 1 (ALC1) protein. J Biol Chem, 2013-08-06;288(45):32357-69. 2013-08-06 [PMID: 23926104]
• Fenton A, Shirodkar P, Macrae C, Meng L, Koch C The PARP3- and ATM-dependent phosphorylation of APLF facilitates DNA double-strand break repair. Nucleic Acids Res, 2013-02-28;41(7):4080-92. 2013-02-28 [PMID: 23449221]
• Yan Q, Xu R, Zhu L, Cheng X, Wang Z, Manis J, Shipp M BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8. Mol Cell Biol, 2012-12-10;33(4):845-57. 2012-12-10 [PMID: 23230272]
• Jaspers J, Kersbergen A, Boon U, Sol W, van Deemter L, Zander S, Drost R, Wientjens E, Ji J, Aly A, Doroshow J, Cranston A, Martin N, Lau A, O'Connor M, Ganesan S, Borst P, Jonkers J, Rottenberg S Loss of 53BP1 causes PARP inhibitor resistance in Brca1-mutated mouse mammary tumors. Cancer Discov, 2012-10-25;3(1):68-81. 2012-10-25 [PMID: 23103855]