Papain Antibody - BSA Free

Western Blot: Papain Antibody [NBP2-44229] - Analysis using the Biotin conjugate of NBP2-44229. Detection of Lane 1: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40

Western Blot: Papain Antibody [NBP2-44229] - lane 1: Papain. Load: 50 ng per lane.

• Reactivity: Pl
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

Papain Antibody - BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya. (Uniprot: P00784)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:500-1:3000
  • Western Blot 1:1000-1:5000
• Application Notes: This product has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Reactivity Notes

Cross reactivity against Papain from other sources may occur but has not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Papain Antibody - BSA Free

• Allergen Car p 1
• Papaya proteinase I
• PPI

Background

Papain is a protease derived from the papaya fruit (Carica papaya). It hydrolyses peptides with broad specificity, but shows a preference for large hydrophobic amino acids in the P2 position. It is synthesised as an inactive 40 kDa precursor containing a 107 residue N terminal pro region and a 26 residue signal peptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P00784(Plant)