Reactivity | HuSpecies Glossary |
Applications | HEStain, IHC, IHC-Fr, ISH |
Description | Frozen tissue slides are ideal for rapid cellular localization of RNA and protein expression, especially for antibodies that don't recognize paraffin embedded tissue. Tissues were snap frozen in liquid nitrogen immediately after excision, and embedded in OCT. The tissue sections are 5-10um in thickness, mounted on positively charged glass slides, and fixed by cold acetone. At least one of the tissue slides from each lot was stained with H & E for quality assurance. |
Dilutions |
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Application Notes | Each individual tissue spot should be >90% core retention and total core lost from 1 slide should be no more than 5% |
Storage | Store at -70C. Avoid freeze-thaw cycles. |
COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme... Read full blog post. |