p38 MAP Kinase Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IL-1 beta (Catalog # 201-LB) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/ Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

p38 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with ultraviolet light (UV), loaded at 0.5 mg/mL. A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Product Discontinued

p38 MAP Kinase Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human, rat and mouse p38 alpha T180/Y182 site
• Specificity: Detects human, mouse and rat Phospho-p38 alpha MAP Kinase when dually phosphorylated at T180/Y182. May also recognize the related phosphorylated isoforms p38 beta , p38 gamma and p38δ.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK14
• Purity Statement: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p38 MAP Kinase Antibody [Unconjugated]

• p38

Background

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p38 MAP Kinase Antibody (AF869)(15)

Publications using AF869

• Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury iScience 2023-06-16 [PMID: 37332598] (Western Blot, Mouse)
• MPO Almeida, CM Mota, TWP Mineo, EAV Ferro, BF Barbosa, NM Silva Heme Oxygenase-1 Induction in Human BeWo Trophoblast Cells Decreases Toxoplasma gondii Proliferation in Association With the Upregulation of p38 MAPK Phosphorylation and IL-6 Production Frontiers in Microbiology, 2021-04-12;12(0):659028. 2021-04-12 [PMID: 33912151] (Western Blot, Human)
• Schlomann U, Koller G, Conrad C, Ferdous T, Golfi P, Garcia A, Hofling S, Parsons M, Costa P, Soper R, Bossard M, Hagemann T, Roshani R, Sewald N, Ketchem R, Moss M, Rasmussen F, Miller M, Lauffenburger D, Tuveson D, Nimsky C, Bartsch J ADAM8 as a drug target in pancreatic cancer. Nat Commun, 2015-01-28;6(0):6175. 2015-01-28 [PMID: 25629724] (Blocking, Human)
• Gutsaeva , Diana R, Montero-Huerta , Pedro, Parkerson , James B, Yerigenahally , Shobha D, Ikuta , Tohru, Head , C Alvin Molecular mechanisms underlying synergistic adhesion of sickle red blood cells by hypoxia and low nitric oxide bioavailability. Blood, 2014-01-15;123(12):1917-26. 2014-01-15 [PMID: 24429338] (Western Blot, Human)
• Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis. PLoS ONE, 2013-11-22;8(11):e80971. 2013-11-22 [PMID: 24278357] (Western Blot, Human)
• Song Z, Liu C, Iwata J, Gu S, Suzuki A, Sun C, He W, Shu R, Li L, Chai Y, Chen Y Mice with Tak1 deficiency in neural crest lineage exhibit cleft palate associated with abnormal tongue development. J Biol Chem, 2013-03-04;288(15):10440-50. 2013-03-04 [PMID: 23460641] (IHC-P, Mouse)
• Altieri P, Spallarossa P, Barisione C, Garibaldi S, Garuti A, Fabbi P, Ghigliotti G, Brunelli C Inhibition of Doxorubicin-Induced Senescence by PPARdelta Activation Agonists in Cardiac Muscle Cells: Cooperation between PPARdelta and Bcl6. PLoS ONE, 2012-09-25;7(9):e46126. 2012-09-25 [PMID: 23049957] (Western Blot, Rat)
• Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 2012-05-23;13(0):50. 2012-05-23 [PMID: 22621398] (Western Blot, Rat)
• Tang WH, Stitham J, Gleim S, Di Febbo C, Porreca E, Fava C, Tacconelli S, Capone M, Evangelista V, Levantesi G, Wen L, Martin K, Minuz P, Rade J, Patrignani P, Hwa J Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane. J. Clin. Invest., 2011-10-17;121(11):4462-76. 2011-10-17 [PMID: 22005299] (Western Blot, Human)
• Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer. Nature, 2010-09-29;468(7320):98-102. 2010-09-29 [PMID: 20881962] (Western Blot, Mouse)
• Lane AE, Tan JT, Hawkins CL The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages. Biochem. J., 2010-08-15;430(1):161-9. 2010-08-15 [PMID: 20528774] (Western Blot, Mouse)
• Saulle E, Riccioni R, Coppola S, Parolini I, Diverio D, Riti V, Mariani G, Laufer S, Sargiacomo M, Testa U Colocalization of the VEGF-R2 and the common IL-3/GM-CSF receptor beta chain to lipid rafts leads to enhanced p38 activation. Br. J. Haematol., 2009-02-24;145(3):399-411. 2009-02-24 [PMID: 19245429] (Western Blot, Human)
• Demuth T, Reavie LB, Rennert JL, Nakada M, Nakada S, Hoelzinger DB, Beaudry CE, Henrichs AN, Anderson EM, Berens ME MAP-ing glioma invasion: mitogen-activated protein kinase kinase 3 and p38 drive glioma invasion and progression and predict patient survival. Mol. Cancer Ther., 2007-04-03;6(4):1212-22. 2007-04-03 [PMID: 17406030] (IHC, Rat)
• Hershko T, Korotayev K, Polager S, Ginsberg D E2F1 modulates p38 MAPK phosphorylation via transcriptional regulation of ASK1 and Wip1. J. Biol. Chem., 2006-08-15;281(42):31309-16. 2006-08-15 [PMID: 16912047] (Western Blot, Human)
• Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2. Cell. Signal., 2005-03-01;17(3):355-63. 2005-03-01 [PMID: 15567066] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MAPK14