Oval Cell Marker Antibody (OC2-4E8) [Alexa Fluor™ Plus 647]

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clone: OC2-4E8
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 647

Oval Cell Marker Antibody (OC2-4E8) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Nonparenchymal cells from DDC-treated mice to induce the oval cell response.
• Localization: Cell surface
• Marker: Hepatic periductal cell Marker
• Specificity: Specific for both proliferating and normal hepatic periductal cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Background

Facultative liver stem cell activation was originally described in the rat, where chemical damage and partial hepatectomy caused the emergence of a new cell type, the oval cell. These cells are thought to be bipotential progenitors capable of yielding both hepatocytes and bile ducts (1). To properly determine the molecular regulation of the progenitor process, appropriate markers are needed to identify and isolate cells that emerge during the oval cell response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.