ORF73/HHV8 Antibody (LN53) [CoraFluor™ 1]

• Reactivity: V-Vi
• Applications: ELISA, IHC
• Clone: LN53
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

ORF73/HHV8 Antibody (LN53) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Recombinant protein corresponding to the latent nuclear antigen-1 molecule of HHV8.
• Localization: Nuclear
• Specificity: This antibody reacts with ORF-73 of human Herpes virus 8 (HHV8). HHV8 is the likely etiological agent of Kaposi's sarcoma (KS). HHV8 encodes a latent nuclear antigen (LNA) which is the product of the viral gene orf 73. LNA is capable of forming a complex with retinoblastoma susceptibility gene product which may be related to its oncogenic activity.
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Rat
• Gene: HHV8GK18_gp81
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for ORF73/HHV8 Antibody (LN53) [CoraFluor™ 1]

• HHV8GK18_gp81
• LANA
• LANA1
• LNA
• ORF73

Background

HHV8 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for HHV8 in kaposi's sarcoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HHV8GK18_gp81