Oligodendrocyte Marker O1 Antibody (O1) [Unconjugated]

Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

Oligodendrocyte Marker O1 was detected in immersion fixed 7 day differentiated rat cortical stem cells using Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: O1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Oligodendrocyte Marker O1 Antibody (O1) [Unconjugated] Summary

• Immunogen: Bovine brain corpus callosum white matter
• Specificity: Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oligodendrocyte Marker O1 Antibody (O1) [Unconjugated]

• Marker
• Oligodendrocyte Marker O1

Background

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
3. Sontheimer, H. et al. (1989) Neuron 2:1135.
4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
7. Cai, Z. et al. (2001) Brain Res. 898:126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oligodendrocyte Marker O1 Antibody (MAB1327)(19)

Publications using MAB1327

• Omri Zveik, Nina Fainstein, Ariel Rechtman, Nitzan Haham, Tal Ganz, Iris Lavon et al. Cerebrospinal fluid of progressive multiple sclerosis patients reduces differentiation and immune functions of oligodendrocyte progenitor cells Glia 2022-06-01 [PMID: 35266197]
• T Arad, N Fainstein, S Goldfarb, M Lachish, T Ganz, T Ben-Hur CD200 -dependent and -independent immune-modulatory functions of neural stem cells Stem Cell Research, 2021-10-07;56(0):102559. 2021-10-07 [PMID: 34634761] (IF, Mouse)
• KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler Donor cell memory confers a metastable state of directly converted cells Cell Stem Cell, 2021-04-12;0(0):. 2021-04-12 [PMID: 33848472] (ICC, Mouse)
• Hyang-Hee Seo, Hyo-Won Han, Sang-Eun Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang Cheol Kim et al. Modelling Toxoplasma gondii infection in human cerebral organoids Emerging Microbes & Infections 2020-09-06 [PMID: 32820712]
• L Hayden, T Semenoff, V Schultz, SF Merz, KJ Chapple, M Rodriguez, AE Warrington, X Shi, CS McKimmie, JM Edgar, K Thümmler, C Linington, M Pingen Lipid-specific IgMs induce antiviral responses in the CNS: implications for progressive multifocal leukoencephalopathy in multiple sclerosis Acta Neuropathol Commun, 2020-08-13;8(1):135. 2020-08-13 [PMID: 32792006] (Bioassay, ICC, Neutralization, Mouse, Rat)
• T Hoshino, H Yamakado, R Takahashi, SI Matsuzawa Susceptibility to erastin-induced ferroptosis decreases during maturation in a human oligodendrocyte cell line FEBS Open Bio, 2020-07-30;0(0):. 2020-07-30 [PMID: 32608563] (ICC, Human)
• Huan Xu, Yulia Dzhashiashvili, Ankeeta Shah, Rejani B. Kunjamma, Yi-lan Weng, Benayahu Elbaz et al. m6A mRNA Methylation Is Essential for Oligodendrocyte Maturation and CNS Myelination Neuron 2020-01-01 [PMID: 31901304]
• Miae Jang, Elizabeth Gould, Jie Xu, Eun Jung Kim, Jun Hee Kim Oligodendrocytes regulate presynaptic properties and neurotransmission through BDNF signaling in the mouse brainstem eLife 2019-04-18 [PMID: 30998186]
• RM Marton, Y Miura, SA Sloan, Q Li, O Revah, RJ Levy, JR Huguenard, SP Pa?ca Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures Nat. Neurosci., 2019-01-28;0(0):. 2019-01-28 [PMID: 30692691] (ICC, Human)
• S Gurung, N Reuter, A Preno, J Dubaut, H Nadeau, K Hyatt, K Singleton, A Martin, WT Parks, JF Papin, DA Myers Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon PLoS Pathog., 2019-01-18;15(1):e1007507. 2019-01-18 [PMID: 30657788] (IHC-Fr, Primate - Papio anubis (Olive Baboon))
• Donna L. McPhie, Ralda Nehme, Caitlin Ravichandran, Suzann M. Babb, Sulagna Dia Ghosh, Alexandra Staskus et al. Oligodendrocyte differentiation of induced pluripotent stem cells derived from subjects with schizophrenias implicate abnormalities in development Translational Psychiatry 2018-10-23 [PMID: 30352993]
• X Yu, G Cheng, L Zhang, Y Zhang, Q Wang, M Zhao, L Zeng, Y Hu, L Feng N-Phenylquinazolin-2-amine Yhhu4952 as a novel promotor for oligodendrocyte differentiation and myelination Sci Rep, 2018-09-19;8(1):14040. 2018-09-19 [PMID: 30232349] (ICC, Human)
• Yi Sun, Yingping Deng, Mili Xiao, Lan Hu, Zhihua Li, Chao Chen Chondroitin sulfate proteoglycans inhibit the migration and differentiation of oligodendrocyte precursor cells and its counteractive interaction with laminin International Journal of Molecular Medicine 2017-09-27 [PMID: 29039438]
• Peddagangannagari Sreekanthreddy, Radka Gromnicova, Heather Davies, James Phillips, Ignacio A. Romero, David Male A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions F1000Research 2015-11-17 [PMID: 26870320]
• Robinson A, Rodgers J, Goings G, Miller S Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis. PLoS ONE, 2014-09-23;9(9):e107649. 2014-09-23 [PMID: 25247590] (Flow Cytometry, Mouse)
• Camille Fung, Xingrao Ke, Ashley S. Brown, Xing Yu, Robert A. McKnight, Robert H. Lane Uteroplacental insufficiency alters rat hippocampal cellular phenotype in conjunction with ErbB receptor expression Pediatric Research 2012-07-01 [PMID: 22367251]
• He LF, Chen HJ, Qian LH, Chen GY, Buzby JS Curcumin protects pre-oligodendrocytes from activated microglia in vitro and in vivo. Brain Res., 2010-04-24;1339(0):60-9. 2010-04-24 [PMID: 20403340] (ICC, Rat)
• Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW Efficient induction of oligodendrocytes from human embryonic stem cells. Stem Cells, 2006-10-19;25(2):419-24. 2006-10-19 [PMID: 17053214] (ICC, Human)
• Rosin C, Bates TE, Skaper SD Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms. J. Neurochem., 2004-09-01;90(5):1173-85. 2004-09-01 [PMID: 15312172] (ICC, Human)