Oligodendrocyte Marker O1 Antibody (O1) [Alexa Fluor® 647]

• Applications: Flow
• Clone: O1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Oligodendrocyte Marker O1 Antibody (O1) [Alexa Fluor® 647] Summary

• Immunogen: Bovine brain corpus callosum white matter
• Specificity: Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.
• Reconstitution Instructions: Reconstitution default not known

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
3. Sontheimer, H. et al. (1989) Neuron 2:1135.
4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
7. Cai, Z. et al. (2001) Brain Res. 898:126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.