Nucleoli Marker Antibody (NM95)

Immunocytochemistry/Immunofluorescence: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleoli Marker Antibody (NM95).

Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleoli Marker Antibody (NM95)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Staining of human colon ca.

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleoli Marker Antibody (NM95).

Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Flow Cytometry of human nucleolar Ag on 293T cells Black: cells alone: Grey: isotype control: Green: AF488-labeled human Nucleolar Monoclonal antibody (NM95)

Flow (Intracellular): Nucleoli Marker Antibody (NM95) [NBP2-32886] - An intracellular stain was performed on Jurkat cells with p27/Kip1 Antibody (KIP1/769) NBP2-47769PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

• Reactivity: Hu, Bv(-), Mu(-), Rt(-)
• Applications: Flow, ICC/IF, IHC
• Clone: NM95
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Nucleoli Marker Antibody (NM95) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34695)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Nuclei of myeloid leukemia biopsy cells
• Localization: Nucleoli
• Marker: Marker For Human Cells
• Specificity: This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.25-0.5 ug/ml
• Application Notes: Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.
  Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.