Nitrotyrosine Antibody (11C2)

• Reactivity: All
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 11C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Nitrotyrosine Antibody (11C2) Summary

• Description: Novus Biologicals Mouse Nitrotyrosine Antibody (11C2) (NB100-57383) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: KLH-Nitrotyrosine
• Specificity: The mouse monoclonal reacts with free and bound Nitrotyrosine
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:500

Reactivity Notes

There is no cross reaction with free or bound Tyrosine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Cell culture supernatant
• Preservative: 0.02% Sodium Azide
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Nitrotyrosine Antibody (11C2)

• Nitrotyrosine

Background

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.