Nitrotryptophan Antibody (4F8)

Western Blot: Nitrotryptophan Antibody (4F8) [NBP2-59355] - Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8 (NBP2-59355). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (1 ug). Lane 4: 6-Nitrotryptophan-BSA (0.5 ug). Lane 5: 6-Nitrotryptophan-BSA (1 ug). Lane 6: 7-Ketocholesterol-BSA (0.5 ug). Lane 7: 7-Ketocholesterol-BSA (1 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody (NBP2-59355) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence: Nitrotryptophan Antibody (4F8) [NBP2-59355] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8 (NBP2-59355). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody (NBP2-59355) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Immunohistochemistry: Nitrotryptophan Antibody (4F8) [NBP2-59355] - Immunohistochemistry analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8 (NBP2-59355). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody (NBP2-59355) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nitrotryptophan Antibody (4F8) Summary

• Immunogen: Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Nitrotryptophan Antibody (4F8)

• 6-Nitrotryptophan;6-NO2-Trp;Nitrotryptophan;NW

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.