In vitro assay: Mouse NFkB Secreted Alkaline Phosphatase SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26261] - mRNA expression patterns of Toll-like receptors in RAW264.7 cells. Total RNAs were prepared and ...read more
Ligand Activation: Mouse NFkB Secreted Alkaline Phosphatase SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26261] - TLR ligand stimulation assay. The RAW cell line was plated in 96-well plates at 5 x 10^4 ...read more
The RAW reporter cell line can be used for screening of TLR agonists or antagonists as well as inhibitory TLR antibody assay. RAW 264.7 cells are known to respond to most Toll-like receptor (TLR) ligands, which trigger the NF-kB induction and lead to inflammatory cytokine production. RT-PCR tests also shows that RAW 264.7 cells produce all of the TLR mRNAs except for TLR5 (Figure 1). Using a 96-well plate format assay, the RAW cell line has been validated by TLR ligand stimulation in which all of the TLR ligands except for TLR5 ligand activated the cell line (Figure 2).
Contents: 3~4 x 10^6 cells
Biosafety Level: 2
The RAW reporter stable cell line is a stably transfected RAW 264.7 cell line which expresses the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.
Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed. - Use pipette aids to prevent ingestion and keep aerosols down to a minimum. - No eating, drinking or smoking while handling the RAW line. - Wash hands after handling the RAW line and before leaving the lab. - Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. - All waste should be considered hazardous. - Dispose of all liquid waste after each experiment and treat with bleach.
Alternate Names for Array
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.
Publications for NFkB Secreted Alkaline Phosphatase Reporter Reporter Cell Line (NBP2-26261)(5)
We have publications tested in 1 application: In vitro.