NFkB Secreted Alkaline Phosphatase Reporter Products

Description

Placental alkaline phosphatase (PLAP) is one of the most stable isoenzymes, only existing in the placenta of higher primates. These characteristics make placental alkaline phosphatase suitable to use as a reporter gene for the analysis of promoter activity and gene expression in cell culture and animal serum. The natural form of PLAP is membrane anchored. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function. SEAP is designed by inserting a translational terminator after amino acid 489 (Berger, et al., Gene 66 (1): 10 (1988). This mutation converts the membrane-bound PLAP protein into the secreted protein SEAP. As a major transcription factor NF-KB plays a key role in regulating genes responsible for the innate and adaptive immune responses. In unstimulated cells, the NF-KB dimers are held in cytoplasm by IKBs that masks the nuclear localization signals of NF-KB. Upon cell stimulation, which leads to IKB degradation, NF-KB quickly translocates to the nucleus and activates various genes that have DNA-binding sites for NF-KB. Our NF-KB SEAPORTER Assay Kit is designed to measure NF-KB activation using SEAP protein secreted to the culture media as a read-out.

Bioinformatics

Alternate Names

SEAP assay