Ligand Activation: Human NFkB Secreted Alkaline Phosphatase SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26260] - Evaluation of the functional activity of the NF-kB/SEAP Stable Reporter Cell Line. ...read more
Human NFkB Secreted Alkaline Phosphatase Reporter SEAP - (SEAPorter™) Stable Reporter Cell Line Summary
Placental alkaline phosphatase is one of the most stable isoenzyme, only existing in the placenta of higher primates. These characteristics make placental alkaline phosphatase suitable to use as a reporter gene for the analysis of promoter activity and gene expression in cell culture and animal serum. The natural form of placental alkaline phosphatase (PLAP) is membrane anchored. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function. SEAP is created by inserting a translational terminator after amino acid 489 (Berger, et al., Gene 66 (1): 10 (1988). This mutation converts the membrane-bound PLAP protein into the secreted protein.
As a major transcription factor, NF-kB plays a key role in regulating genes responsible for the innate and adaptive immune responses. In unstimulated cells, the NF-kB dimers are held in the cytoplasm by IkBs that masks the nuclear localization signals of NF-kB. Upon cell stimulation, which leads to IkB degradation, NF-kB quickly translocates to the nucleus and activates various genes that have DNA-binding sites for NF-kB.
The NF-kB/SEAP stable HEK 293 cell line is designed to measure NF-kB activation using SEAP protein secreted to the culture media as a read-out with our SEAPorter™ Assay kit (NBP2-25285). The NF-kB/SEAP stable cells are not only useful in helping with the identification of pro or anti-inflammatory substances, but also can help to assay for proteasome activity since the activation of NF-kB results in the degradation of IkB through the proteasome-dependant pathway.
Contents: 3~4 x 10^6 cells
Biosafety Level: 2
The NF-kB/SEAP is a reporter gene assay for the detection of NF-kB activation and contains a cell line modified for this purpose. This cell line is derived from HEK 293 (Human Embryonal Kidney) cells. It is stably transfected with the SEAP (secreted alkaline phosphatase) reporter gene under the transcriptional control of an NF-kB response element. The recombinant cell line is provided frozen.
Details for NFkB Secreted Alkaline Phosphatase Reporter
Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed. - Use pipette aids to prevent ingestion and keep aerosols down to a minimum. - No eating, drinking or smoking while handling cells. - Wash hands after handling cultures and before leaving the lab. - Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells. - All waste should be considered hazardous. - Dispose of all liquid waste after each experiment and treat with bleach.
Alternate Names for NFkB Secreted Alkaline Phosphatase Reporter
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.
Publications for NFkB Secreted Alkaline Phosphatase Reporter Reporter Cell Line (NBP2-26260)(5)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: ELISA, In vitro.