N OLLAS Epitope Tag Expression and Detection Kit

Images

 
Western Blot: N OLLAS Epitope Tag Kit [NBK1-18296] - Western Blot on OLLAS tagged SFO-p41 protein.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IP

Order Details

N OLLAS Epitope Tag Expression and Detection Kit Summary

Kit Type
Expression and Detection Kit

Applications/Dilutions

Application Notes
Flow Cytometry was reported in scientific literature.
Publications
Read Publications using NBK1-18296.

Reactivity Notes

Tag specific

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Alternate Names for N OLLAS Epitope Tag Expression and Detection Kit

  • OLLA-2

Background

The pCMV-N-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with an N-terminal OLLAS tag. Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for N OLLAS Epitope Tag Kit (NBK1-18296)(2)

We have publications tested in 5 applications: FLOW, ICC/IF, IHC-Fr, IP, WB.


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FLOW
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ICC/IF
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IHC-Fr
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IP
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional N OLLAS Epitope Tag Products

Array NBK1-18296

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