N-Me-6,7-diOH-TIQ Antibody (106814) [Unconjugated]

• Reactivity: Multi
• Applications: IHC
• Clone: 106814
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

N-Me-6,7-diOH-TIQ Antibody (106814) [Unconjugated] Summary

• Immunogen: Carrier protein-coupled N‑Me‑6,7-diOH-TIQ
• Specificity: Recognizes N‑Me‑6,7‑diOH‑TIQ in immunoassays. The specificity was determined by a competition type assay where the N‑Me‑6,7‑diOH‑TIQ labeled with biotin at position 5 or 8 competed with another compound for binding to the antibody binding site. The detection limit of biotinylated N‑Me‑6,7‑diOH‑TIQ in assays where the molecule was captured by the antibody coated on microplates and developed by HRP‑streptavidin was 5 nM. The dissociation constants of the interaction between the antibody and N‑Me‑6,7‑diOH‑TIQ and its biotinylated analog were 0.2 and 0.5 µM, respectively. This antibody specifically recognizes N‑Me‑6,7‑diOH‑TIQ and shows 60% cross‑reactivity with 6,7‑diOH‑TIQ, 15% cross‑reactivity with 1,N‑di‑Me‑6,7‑diOH‑TIQ, 3% cross‑reactivity with 1‑Me‑6,7‑diOH‑TIQ, and less than 1% or no cross‑reactivity with tetrahydroisoquinoline (TIQ), 5‑OH‑isoquiniline, 5‑OH‑N‑Me‑isoquinolinium, tetrahydropapaveroline, 9H‑pyrido[3,4‑b]indole, and 1,2,3,4‑tetrahydro‑9H‑pyrido[3,4‑b]indole. The percent cross‑reactivity is expressed by the relative value, which produced 50% inhibition of interaction between the biotin‑labeled N‑methyl‑6,7‑dihydroxytetrahydroisoquinoline and the other compound.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for N-Me-6,7-diOH-TIQ Antibody (106814) [Unconjugated]

• 7diOHTIQ
• NMe6
• N-Me-6,7-diOH-TIQ

Background

Tetrahydroisoquinolines (TIQs) are structurally similar to MPTP, an agent known to cause Parkinsonism. TIQs may play a role in pathogenesis of Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.