Mycobacterium Tuberculosis L-Alanine Dehydrogenase Antibody (1C3) [Alexa Fluor® 750]

Product Discontinued

Mycobacterium Tuberculosis L-Alanine Dehydrogenase Antibody (1C3) [Alexa Fluor® 750] Summary

• Immunogen: Culture filtrat from Mycobacterium tuberculosis H37Rv
• Epitope: recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis (4).
• Specificity: Specific for a 40 kDa band from short term culture of Mycobacterium tuberculosis in Western blotting after SDS-PAGE. The 40 kDa band was identified as the protein L-alanine dehydrogenase (Ald). Interspecies analyses with HBT 10 showed reacts with M. tuberculosis and M. marinum but not M. bovis BCG.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mycobacterium Tuberculosis L-Alanine Dehydrogenase Antibody (1C3) [Alexa Fluor® 750]

• 40 kDa antigen
• Alanine dehydrogenase
• EC 1.4.1.1
• MT2850
• MTV002.45
• Rv2780
• TB43

Background

Mycobacterium L-alanine dehydrogenase (ald) belongs to the AlaDH/PNT family and is a 40 kDa secreted protein from short term culture of Mycobacterium tuberculosis; which may play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.