Mycobacterium Tuberculosis L-Alanine Dehydrogenase Antibody (1C3) - BSA Free

• Reactivity: Ba
• Applications: WB, ELISA
• Clone: 1C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Mycobacterium Tuberculosis L-Alanine Dehydrogenase Antibody (1C3) - BSA Free Summary

• Immunogen: Culture filtrat from Mycobacterium tuberculosis H37Rv
• Epitope: recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis (4).
• Specificity: Specific for a 40 kDa band from short term culture of Mycobacterium tuberculosis in Western blotting after SDS-PAGE. The 40 kDa band was identified as the protein L-alanine dehydrogenase (Ald). Interspecies analyses with HBT 10 showed reacts with M. tuberculosis and M. marinum but not M. bovis BCG.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Mycobacterium Tuberculosis L-Alanine Dehydrogenase Antibody (1C3) - BSA Free

• 40 kDa antigen
• Alanine dehydrogenase
• EC 1.4.1.1
• MT2850
• MTV002.45
• Rv2780
• TB43

Background

Mycobacterium L-alanine dehydrogenase (ald) belongs to the AlaDH/PNT family and is a 40 kDa secreted protein from short term culture of Mycobacterium tuberculosis; which may play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.