Recombinant Mycobacterium tuberculosis Ab85A His Protein

SDS-Page: TB Ab85A Protein [NBP1-99035]

• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Mycobacterium tuberculosis Ab85A His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 43-338 of Mycobacterium tuberculosis TB Ab85A
  
  

  Source:Baculovirus

  Amino Acid Sequence: ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH

• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 32.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mycobacterium tuberculosis

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mycobacterium tuberculosis Ab85A His Protein

• 85 A
• 85A
• Ag85A
• Antigen 85 A
• Antigen 85 complex A
• Antigen 85A
• Fbp A
• FbpA
• Fibronectin Binding protein A
• Mpt 44
• Mpt44
• Mycobacterium tuberculosis antigen 85A
• Mycolyl transferase 85 A
• Mycolyl transferase 85A
• Rv3804c
• Secreted antigen Ag85A

Background

Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Mycobacterium tuberculosis Ab85A Recombinant Protein (NBP1-99035)(1)

Publication using NBP1-99035

• Shurygina AP, Zabolotnykh N, Vinogradova T et al. Preclinical Evaluation of TB/FLU-04L-An Intranasal Influenza Vector-Based Boost Vaccine against Tuberculosis International journal of molecular sciences 2023-04-18 [PMID: 37108602] (In vitro assay)

FAQs for Mycobacterium tuberculosis Ab85A Recombinant Protein (NBP1-99035). (Showing 1 - 1 of 1 FAQ).

1. Is this product useful for T cell stimulation assays?
   • The TB Ab85A Protein (NBP1-99035) has only been validated in SDS-PAGE. It is a recombinant protein (with a His-tag), and it has not been tested for any functional activity. Therefore, unfortunately, we do not know whether or not it would work in T cell stimulation assays, and this application is not covered by our guarantee. However, if you do test this recombinant protein in your functional assay, you could apply for our Innovator's Reward program, where in return for sharing your results (positive or negative) with us by submitting an online review, you would receive a discount voucher for 100% of the purchase price of the reviewed product. For more information, please contact us at innovators@novusbio.com for more information.