Monocyte/Macrophage Antibody (KUL01)

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Flow Cytometry: Monocyte/Macrophage Antibody (KUL01) [NBP1-28359] - Analysis using the FITC conjugate of NBP1-28359. Multiple staining of peripheral blood mononuclear cells.

Product Details

Summary
Reactivity ChSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, ICC
Clone
KUL01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Catalog# & Conjugate Size Price

Monocyte/Macrophage Antibody (KUL01) Summary

Immunogen
Chicken peripheral blood mononuclear cell leukocytes
Specificity
Chicken monocytes and macrophages
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:200
  • Immunohistochemistry-Frozen 1:10 - 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry
Application Notes
Flow Cytometry and Immunohistochemistry. Although not tested this antibody may be useful in IHC.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Monocyte/Macrophage Antibody (KUL01)

  • macrophage
  • macrophages
  • monocyte
  • monocytes

Background

The monoclonal antibody KUL01 is useful in the study of the development, distribution, function and ontogeny of the mononuclear phagocyte system (MPS) of the chicken by exclusively recognizing the cells of the MPS. It identifies chicken monocytes and macrophages as well as interdigitating cells and activated microglia cells. This antibody does not react with B (Bu-1+) or T (CD3+) lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Monocyte/Macrophage Antibody (NBP1-28359). (Showing 1 - 1 of 1 FAQ).

  1. Is there a protocol available for the use of this antibody in parrafin embedded chicken tissue slides?
    • Unfortunately, we did not use any customized protocol for validating this product, however, please refer to our general IHC protocol. We recommend a dilution range of 1:10-1:2000 of the primary antibody for IHC. Moreover, we would encourage you to follow the research publication by the inventor of this antibody - Mast et al. 1998 (Vet Immunol Immunopathol. 61(2-4):343-57), wherein this product was validated for FLOW and IHC applications.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

FITC Labeled NBP1-28360

Additional Monocyte/Macrophage Products

Monocyte/Macrophage NBP1-28359

Blogs on Monocyte/Macrophage

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