M.O.M.™ Staining Kit (Fluorometric)

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Product Details

Summary
Applications ICC/IF, IHC, IHC-Fr, IHC-P

Order Details

M.O.M.™ Staining Kit (Fluorometric) Summary

Description
Key advantages of the Vector® M.O.M.™ Staining Kits
  • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
  • Clear, crisp specific staining of antigens of interest
  • Based on VECTASTAIN® ABC reagent protocols
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required
  • Kit Type
    Staining Kit (Fluorometric)

    Applications/Dilutions

    Application Notes
    This Vector(R) M.O.M.(TM) kit contains sufficient stock reagents to provide approximately 25 ml of working solution which is usually enough to stain about 125-250 mouse tissue sections.

    Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.

    Kit Components

    Components
    1. 0.1 ml M.O.M.™Biotinylated Anti-Mouse IgG Reagent
    2. 0.4 ml Fluorescein Avidin DCS
    3. 1 ml M.O.M.™ Mouse Ig Blocking Reagent
    4. 6 ml of M.O.M.™ Protein Concentrate

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    The Vector(R) M.O.M.(TM) Immunodetection Kit is designed specifically to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. The background staining caused by the presence of endogenous mouse IgG can be essentially eliminated by using the Vector(R) M.O.M.(TM) Immunodetection Kit. The M.O.M.(TM) Kit utilizes a novel blocking agent and a special detection methodology to significantly reduce this undesired background staining. The Vector(R) M.O.M.(TM) Kit can be used with normal and genetically engineered mouse models, including transgenic, xenograft, knock out and other mutant strains.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

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    Other Available Formats

    Additional M.O.M.(TM) Products

    Array FMK-2201-NB

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