Applications: ICC/IF, IHC
Description
The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit is designed specifically to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. The background staining caused by the presence of endogenous mouse IgG can be essentially eliminated by using the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit. The M.O.M.(R) (Mouse-On-Mouse) Kit utilizes a novel blocking agent and a special detection methodology to significantly reduce this undesired background staining. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit can be used with normal and genetically engineered mouse models, including transgenic, xenograft, knock out and other mutant strains.
