M.O.M.® (Mouse-On-Mouse) Staining Kit (Fluorometric)

• Applications: ICC/IF, IHC

M.O.M.® (Mouse-On-Mouse) Staining Kit (Fluorometric) Summary

• Description: Key advantages of the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Staining Kit (Fluorometric)
  Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
  Clear, crisp specific staining of antigens of interest
  Based on VECTASTAIN(R) ABC reagent protocols
  Procedures are simple and easy to follow
  No tedious calculations or antibody prebinding steps required
• Kit Type: Staining Kit (Fluorometric)

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: This Vector(R) M.O.M.(R) (Mouse-On-Mouse) kit contains sufficient stock reagents to provide approximately 25 ml of working solution which is usually enough to stain about 125-250 mouse tissue sections..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit is designed specifically to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. The background staining caused by the presence of endogenous mouse IgG can be essentially eliminated by using the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit. The M.O.M.(R) (Mouse-On-Mouse) Kit utilizes a novel blocking agent and a special detection methodology to significantly reduce this undesired background staining. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit can be used with normal and genetically engineered mouse models, including transgenic, xenograft, knock out and other mutant strains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.