M.O.M.® (Mouse-On-Mouse) Elite® IgG HRP Immunodetection Kit

Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB] - Mouse, Newborn (eye, double label): GFAP (m), M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection kit, Vector NovaRED substrate (red); Synapsin (m), MOM Peroxidase Kit, DAB+Ni substrate (gray/black). Note contrast with endogenous pigment (brown) seen in surrounding tissues.

Immunohistochemistry: M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB] - Mouse, Newborn (tongue, double label): Synapsin (m), , Vector NovaRED substrate (red); Desmin (m), M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection kit, DAB+Ni substrate (gray/black).

M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit [PK-2200-NB] - M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection kit

• Reactivity: Mu
• Applications: WB, ICC/IF, IHC

M.O.M.® (Mouse-On-Mouse) Elite® IgG HRP Immunodetection Kit Summary

• Description: Features:
  Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
  Clear, crisp specific staining of antigens of interest
  Based on ImmPRESS(R) reagent protocols
  Procedures are simple and easy to follow
  No tedious calculations or antibody prebinding steps required
• Kit Type: HRP Immunodetection Kit

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: The Vector M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue section.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector(R) M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit. The result is clear, crisp specific staining of the antigens of interest. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Elite(R) IgG HRP Immunodetection Kit contains a proprietary Mouse IgG Blocking Reagent and is based on the simple and easy to follow VECTASTAIN ABC reagent protocols; no tedious calculations or antibody pre-binding steps are required. Excellent staining results for a once difficult application have now become routine with the Vector(R) M.O.M.(R) System.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.