MMTV Antibody

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Western Blot: MMTV Antibody [NBP2-44179] - lane 1: cell lysate negative control. Lane 2: cell lysate spiked with purified virus. Load: 10 Ug per lane.

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MMTV Antibody Summary

Immunogen
MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus surface protein tagged with His. (Uniprot: P03374)
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination
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Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:20000
  • Western Blot 1:1000-1:2000
Application Notes
This product has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 41.2 kDa in size corresponding to MMTV surface protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with MMTV from mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Notes

MMTV surface protein antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01% and cross adsorbed with 6X HIS

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for MMTV Antibody

  • Envelope glycoprotein gp70
  • Glycoprotein 36
  • Glycoprotein 52
  • Mouse mammary tumor virus (strain GR)
  • Surface protein
  • Transmembrane protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Uniprot